GeneTex
United States (US)

Synaptojanin 1 antibody [AC1]

GTX15835_337_ICC-IF_19011519_681.jpg
GTX15835_339_ICC-IF_19011519_362.jpg
GTX15835_1049_IHC-P_19011519_852.jpg
GTX15835_1546_WB_19011519_497.jpg
GTX15835_338_ICC-IF_19011519_461.jpg

Cat No. GTX15835

Host Mouse
Clonality Monoclonal
Clone Name AC1
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:10-1:100
IHC-P 1:100
IHC-Fr 1/20
IP Assay dependent
Not tested in other applications.

Calculated MW

173 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Ascites diluted in PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Proline rich domain of a fusion protein comprising amino acids 1156-1286 of rat synaptojanin 1.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Synaptojanin 1,Synj1

Cellular Localization

Cytoplasm,Perinuclear region

Background

has inositol 5-phosphatase activity; interacts with dynamin to play a role in synaptic vesicle recycling [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
GTX15835_337_ICC-IF_19011519_681.jpg

GTX15835 ICC/IF Image

ICC/IF analysis of C6 cells using GTX15835 Synaptojanin 1 antibody [AC1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

GTX15835_339_ICC-IF_19011519_362.jpg

GTX15835 ICC/IF Image

ICC/IF analysis of U-87 MG cells using GTX15835 Synaptojanin 1 antibody [AC1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

GTX15835_1049_IHC-P_19011519_852.jpg

GTX15835 IHC-P Image

IHC-P analysis of human brain tissue using GTX15835 Synaptojanin 1 antibody [AC1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX15835_1546_WB_19011519_497.jpg

GTX15835 WB Image

WB analysis of 25 ug of C6 cell lysates using GTX15835 Synaptojanin 1 antibody [AC1].
Dilution : 1:500

GTX15835_338_ICC-IF_19011519_461.jpg

GTX15835 ICC/IF Image

ICC/IF analysis of PC12 cells using GTX15835 Synaptojanin 1 antibody [AC1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

Package List Price ($)
$ 319