ICC/IF analysis of C6 cells using GTX15835 Synaptojanin 1 antibody [AC1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

ICC/IF analysis of U-87 MG cells using GTX15835 Synaptojanin 1 antibody [AC1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

ICC/IF analysis of PC12 cells using GTX15835 Synaptojanin 1 antibody [AC1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

WB analysis of 25 ug of C6 cell lysates using GTX15835 Synaptojanin 1 antibody [AC1].
Dilution : 1:500

IHC-P analysis of human brain tissue using GTX15835 Synaptojanin 1 antibody [AC1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100