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Synaptojanin 1 antibody [AC1]

Anti-Synaptojanin 1 antibody [AC1] used in IHC (Paraffin sections) (IHC-P). GTX15835
Anti-Synaptojanin 1 antibody [AC1] used in Western Blot (WB). GTX15835
Anti-Synaptojanin 1 antibody [AC1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15835
Anti-Synaptojanin 1 antibody [AC1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15835
Anti-Synaptojanin 1 antibody [AC1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15835

Cat. No. GTX15835

Host

Mouse

Clonality

Monoclonal

Clone Name

AC1

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:10-1:100
IHC-P 1:100
IHC-Fr 1/20
IP Assay dependent
Not tested in other applications.

Calculated MW

173 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites diluted with PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Proline rich domain of a fusion protein comprising amino acids 1156-1286 of rat synaptojanin 1.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptojanin 1

Background

has inositol 5-phosphatase activity; interacts with dynamin to play a role in synaptic vesicle recycling [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Synaptojanin 1 antibody [AC1] used in IHC (Paraffin sections) (IHC-P). GTX15835

GTX15835 IHC-P Image

IHC-P analysis of human brain tissue using GTX15835 Synaptojanin 1 antibody [AC1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-Synaptojanin 1 antibody [AC1] used in Western Blot (WB). GTX15835

GTX15835 WB Image

WB analysis of 25 ug of C6 cell lysates using GTX15835 Synaptojanin 1 antibody [AC1].
Dilution : 1:500

Anti-Synaptojanin 1 antibody [AC1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15835

GTX15835 ICC/IF Image

ICC/IF analysis of PC12 cells using GTX15835 Synaptojanin 1 antibody [AC1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

Anti-Synaptojanin 1 antibody [AC1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15835

GTX15835 ICC/IF Image

ICC/IF analysis of U-87 MG cells using GTX15835 Synaptojanin 1 antibody [AC1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

Anti-Synaptojanin 1 antibody [AC1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15835

GTX15835 ICC/IF Image

ICC/IF analysis of C6 cells using GTX15835 Synaptojanin 1 antibody [AC1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399