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Synaptophysin antibody [GT1825]

Anti-Synaptophysin antibody [GT1825] used in IHC (Paraffin sections) (IHC-P). GTX633821
Anti-Synaptophysin antibody [GT1825] used in Western Blot (WB). GTX633821
Anti-Synaptophysin antibody [GT1825] used in IHC (Paraffin sections) (IHC-P). GTX633821

Cat. No. GTX633821

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1825

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Synaptophysin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

rat brain

Predict Reactivity

Human, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Synaptophysin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888384

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptophysin , MRX96 , MRXSYP

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Multi-pass membrane protein , Cell junction , synapse , synaptosome

Background

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Synaptophysin antibody [GT1825] used in IHC (Paraffin sections) (IHC-P). GTX633821

GTX633821 IHC-P Image

Synaptophysin antibody [GT1825] detects Synaptophysin protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Synaptophysin antibody [GT1825] (GTX633821) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody [GT1825] used in Western Blot (WB). GTX633821

GTX633821 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody (GTX633821) diluted at 1:3000.

Anti-Synaptophysin antibody [GT1825] used in IHC (Paraffin sections) (IHC-P). GTX633821

GTX633821 IHC-P Image

Synaptophysin antibody [GT1825] detects Synaptophysin protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Synaptophysin antibody [GT1825] (GTX633821) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Goto T et al. J Otolaryngol Head Neck Surg 2023; 52 (1) : 47 Effects of early local administration of high-dose bFGF on a recurrent laryngeal nerve injury model.
Hsieh MC et al. Sci Rep 2016; 6 : 37411 Tet1-dependent epigenetic modification of BDNF expression in dorsal horn neurons mediates neuropathic pain in rats.
Application : IHC-Fr
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations