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Synaptophysin antibody [GT2589]

Comparison
Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972
Anti-Synaptophysin antibody [GT2589] used in IHC (Paraffin sections) (IHC-P). GTX633972
Anti-Synaptophysin antibody [GT2589] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633972
Anti-Synaptophysin antibody [GT2589] used in IHC (Paraffin sections) (IHC-P). GTX633972
Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972
Anti-Synaptophysin antibody [GT2589] used in IHC (Paraffin sections) (IHC-P). GTX633972
Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972
Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972

Cat. No. GTX633972

1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT2589

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Synaptophysin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

rat brain

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Synaptophysin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2885145

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptophysin , MRX96 , MRXSYP

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Multi-pass membrane protein , Cell junction , synapse , synaptosome

Background

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972

GTX633972 WB Image

Comparison

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Synaptophysin antibody [GT2589] used in IHC (Paraffin sections) (IHC-P). GTX633972

GTX633972 IHC-P Image

Synaptophysin antibody [GT2589] detects Synaptophysin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Synaptophysin stained by Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody [GT2589] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633972

GTX633972 ICC/IF Image

Synaptophysin antibody [GT2589] detects Synaptophysin protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptophysin stained by Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Synaptophysin antibody [GT2589] used in IHC (Paraffin sections) (IHC-P). GTX633972

GTX633972 IHC-P Image

Synaptophysin antibody [GT2589] detects Synaptophysin protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972

GTX633972 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Synaptophysin antibody [GT2589] used in IHC (Paraffin sections) (IHC-P). GTX633972

GTX633972 IHC-P Image

Synaptophysin antibody [GT2589] detects Synaptophysin protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972

GTX633972 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000.

Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972

GTX633972 WB Image

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

Synaptophysin antibody [GT2589] Cat. No. GTX633972

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972
Date : Greg Jacobson submitted on 09-Jun-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse brain lysate
Amount used : 1 uL in 5mL
Blocking : 5% milk in TBST, 22°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Lane Description : Lane 1: 10ug extracted protein mouse brain (0.4%NP-40 lysis) 37oC Control. Lane 2: 10ug extracted protein mouse brain (0.4%NP-40 lysis) 37oC + drug. Lane 3: 10ug extracted protein mouse brain (0.4%NP-40 lysis) 43oC Control. Lane 4: 10ug extracted protein mouse brain (0.4%NP-40 lysis) 43oC + drug.
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations