Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 9.0 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Colon: A moderate to strong staining of the axons of the Auerbach’s and Meissner’s plexus should be seen while the endocrine cells of the mucosa and the peripheral nerves in the mucosa must show an at least a weak to moderate staining. Pancreas: A strong staining should be seen in islets of Langerhans and in peripheral nerve fibres located between the exocrine cells.
Negative Control
Colon: All non-endocrine epithelial cells and lymphocytes should not show any staining.
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 274-313) of human Synaptophysin (SYP) protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
synaptophysin , MRX96 , MRXSYP
Cellular Localization
Synapse, synaptosome
Background
This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with an X-linked form of cognitive disability. [provided by RefSeq, Jul 2017]
Database
Research Area
![Anti-Synaptophysin antibody [MSVA-462R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04371](/upload/website/prouct_img/small/GTX04371/GTX04371_20230728_IHC-P_365_23072723_311.webp "Anti-Synaptophysin antibody [MSVA-462R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04371")
![Anti-Synaptophysin antibody [MSVA-462R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04371](/upload/website/prouct_img/small/GTX04371/GTX04371_20230728_IHC-P_105_23072722_201.webp "Anti-Synaptophysin antibody [MSVA-462R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04371")
![Anti-Synaptophysin antibody [MSVA-462R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04371](/upload/website/prouct_img/small/GTX04371/GTX04371_20230728_IHC-P_235_23072723_306.webp "Anti-Synaptophysin antibody [MSVA-462R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04371")
![Anti-Synaptophysin antibody [MSVA-462R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04371](/upload/website/prouct_img/normal/GTX04371/GTX04371_20230728_IHC-P_365_23072723_311.webp "Anti-Synaptophysin antibody [MSVA-462R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04371")
![Anti-Synaptophysin antibody [MSVA-462R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04371](/upload/website/prouct_img/normal/GTX04371/GTX04371_20230728_IHC-P_105_23072722_201.webp "Anti-Synaptophysin antibody [MSVA-462R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04371")
![Anti-Synaptophysin antibody [MSVA-462R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04371](/upload/website/prouct_img/normal/GTX04371/GTX04371_20230728_IHC-P_235_23072723_306.webp "Anti-Synaptophysin antibody [MSVA-462R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04371")