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Synaptophysin antibody [MSVA-462R] HistoMAX

HistoMAX
Anti-Synaptophysin antibody [MSVA-462R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04371
Anti-Synaptophysin antibody [MSVA-462R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04371
Anti-Synaptophysin antibody [MSVA-462R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04371

Cat. No. GTX04371

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 MSVA-462R

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($629)
See all Synaptophysin products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 9.0 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Colon: A moderate to strong staining of the axons of the Auerbach’s and Meissner’s plexus should be seen while the endocrine cells of the mucosa and the peripheral nerves in the mucosa must show an at least a weak to moderate staining. Pancreas: A strong staining should be seen in islets of Langerhans and in peripheral nerve fibres located between the exocrine cells.

Negative Control

Colon: All non-endocrine epithelial cells and lymphocytes should not show any staining.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 274-313) of human Synaptophysin (SYP) protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptophysin , MRX96 , MRXSYP

Cellular Localization

Synapse, synaptosome

Background

This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with an X-linked form of cognitive disability. [provided by RefSeq, Jul 2017]

Database

Research Area

DATA IMAGES

Anti-Synaptophysin antibody [MSVA-462R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04371

GTX04371 IHC-P Image

IHC-P analysis of human thyroid Medullary carcinoma tissue section using GTX04371 Synaptophysin antibody [MSVA-462R] HistoMAX.
Thyroid Medullary carcinoma with strong cytoplasmic synaptophysin immunostaining of all tumor cells.

Anti-Synaptophysin antibody [MSVA-462R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04371

GTX04371 IHC-P Image

IHC-P analysis of human pancreas tissue section using GTX04371 Synaptophysin antibody [MSVA-462R] HistoMAX.
A strong synaptophysin immunostaining is seen in islet cells of Langerhans.

Anti-Synaptophysin antibody [MSVA-462R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04371

GTX04371 IHC-P Image

IHC-P analysis of human cerebellum tissue section using GTX04371 Synaptophysin antibody [MSVA-462R] HistoMAX.
Cerebellum molecular granular and Purkinje layers white matter. Synaptophysin is strongly expressed in cerebellum molecular Purkinje and granular layer.

REFERENCE

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REVIEW

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Package List Price ($)
$ 629