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Synaptophysin antibody

Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in IHC (Frozen sections) (IHC-Fr). GTX100865
Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

Cat. No. GTX100865

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Synaptophysin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038077

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptophysin , MRX96 , MRXSYP

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Multi-pass membrane protein , Cell junction , synapse , synaptosome

Background

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865

GTX100865 ICC/IF Image

Synaptophysin antibody detects Synaptophysin protein at synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptophysin protein stained by Synaptophysin antibody (GTX100865) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.
Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4ºC and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody used in IHC (Frozen sections) (IHC-Fr). GTX100865

GTX100865 IHC-Fr Image

Synaptophysin antibody detects Synaptophysin protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse muscle.
Green: Synaptophysin stained by Synaptophysin antibody (GTX100865) diluted at 1:250.
Red: α-Bungarotoxin, stained by α-Bungarotoxin, Alexa Fluor™ 594 conjugate (B13423) diluted at 1:5000.
Blue: Hoechst 33342 staining.

Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865

GTX100865 ICC/IF Image

Synaptophysin antibody detects Synaptophysin protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptophysin stained by Synaptophysin antibody (GTX100865) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865

GTX100865 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody (GTX100865) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein at on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Synaptophysin antibody (GTX100865) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Synaptophysin stained by Synaptophysin antibody (GTX100865) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865

GTX100865 WB Image

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody (GTX100865) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft , using Synaptophysin(GTX100865) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: Synaptophysin protein stained by Synaptophysin antibody (GTX100865) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Synaptophysin stained by Synaptophysin antibody (GTX100865) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

Synaptophysin antibody Cat. No. GTX100865

Rating ( Average 5 based on 5 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28465562
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28465562
Date : Anonymous submitted on 11-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Brain tissue lysate
Amount used : 20-30 μg
Blocking : 3% milk powder in TBST, 25°C, 1Hr
Primary Antibody : 1:8000, 4°C, 8Hr
Figure Note : Numbers indicated separate mice; L: left and R: right hippocampus and prefrontal cortex (PFC).
Date : Anonymous submitted on 25-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : LNCaP cells
Amount used : 40μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 23-Mar-2012
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Small Intestine tissue
Primary Antibody : 1:200, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169