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Synaptophysin antibody

Orthogonal
Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

Cat No. GTX100865

Reference 7
2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
See all Synaptophysin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

human brain , mouse brain , rat brain , *Mouse small intestine , *LNCaP

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Synaptophysin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

synaptophysin,MRX96,MRXSYP

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Multi-pass membrane protein , Cell junction , synapse , synaptosome

Background

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865

GTX100865 ICC/IF Image

Synaptophysin antibody detects Synaptophysin protein at synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptophysin protein stained by Synaptophysin antibody (GTX100865) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Orthogonal

Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.
Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4℃ and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865

GTX100865 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody (GTX100865) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: Synaptophysin protein stained by Synaptophysin antibody (GTX100865) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865

GTX100865 WB Image

Human tissue extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody (GTX100865) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865

GTX100865 WB Image

Synaptophysin antibody detects SYP protein by western blot analysis.
A. 50 µg rat brain lysate/extract
10% SDS-PAGE
Synaptophysin antibody (GTX100865) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein at on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Synaptophysin antibody (GTX100865) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft , using Synaptophysin(GTX100865) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
REFERENCE

Application Reference

Bilousova T et al. Am J Pathol 2019; (Epub) Apolipoprotein E/Amyloid-帣 Complex Accumulates in Alzheimer Disease Cortical Synapses via Apolipoprotein E Receptors and Is Enhanced by APOE4.
Application : ICC/IF
Reactivity : Human
Donadon I et al. Nucleic Acids Res 2019; (Epub) Rescue of spinal muscular atrophy mouse models with AAV9-Exon-specific U1 snRNA.
Application : IHC-Fr
Reactivity : Mouse
Zhang L et al. Neurogastroenterol Motil 2018; : e13430 Sustained pain hypersensitivity in the stressed colon: Role of mast cell-derived nerve growth factor-mediated enteric synaptic plasticity.
Application : IHC-P
Reactivity : Rat
Pfanzagl B et al. Sci Rep 2017; 7 (1) : 1313 Activation of the ileal neuroendocrine tumor cell line P-STS by acetylcholine is amplified by histamine: role of H3R and H4R.
Chang YT et al. Sci Rep 2017; 7 : 42795 REST is a crucial regulator for acquiring EMT-like and stemness phenotypes in hormone-refractory prostate cancer.
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REVIEW

Synaptophysin antibody Cat No. GTX100865

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865
Date : Anonymous submitted on 25-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : LNCaP cells
Amount used : 40μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Date : Anonymous submitted on 23-Mar-2012
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Small Intestine tissue
Primary Antibody : 1:200, 4°C, 16Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169