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Synaptophysin antibody

Orthogonal
Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in IHC (Frozen sections) (IHC-Fr). GTX100865
Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865
Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865

Cat. No. GTX100865

Reference 22
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all Synaptophysin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Synaptophysin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038077

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptophysin , MRX96 , MRXSYP

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Multi-pass membrane protein , Cell junction , synapse , synaptosome

Background

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865

GTX100865 ICC/IF Image

Synaptophysin antibody detects Synaptophysin protein at synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptophysin protein stained by Synaptophysin antibody (GTX100865) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Orthogonal

Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.
Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4ºC and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody used in IHC (Frozen sections) (IHC-Fr). GTX100865

GTX100865 IHC-Fr Image

Synaptophysin antibody detects Synaptophysin protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse muscle.
Green: Synaptophysin stained by Synaptophysin antibody (GTX100865) diluted at 1:250.
Red: α-Bungarotoxin, stained by α-Bungarotoxin, Alexa Fluor™ 594 conjugate (B13423) diluted at 1:5000.
Blue: Hoechst 33342 staining.

Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865

GTX100865 ICC/IF Image

Synaptophysin antibody detects Synaptophysin protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptophysin stained by Synaptophysin antibody (GTX100865) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865

GTX100865 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody (GTX100865) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein at on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Synaptophysin antibody (GTX100865) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Synaptophysin stained by Synaptophysin antibody (GTX100865) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865

GTX100865 WB Image

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody (GTX100865) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft , using Synaptophysin(GTX100865) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: Synaptophysin protein stained by Synaptophysin antibody (GTX100865) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Synaptophysin stained by Synaptophysin antibody (GTX100865) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865

GTX100865 ICC/IF Image

The data was published in the journal Sci Rep in 2017.PMID: 28465562

Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865

GTX100865 ICC/IF Image

The data was published in the journal Sci Rep in 2017.PMID: 28465562

REFERENCE

Application Reference

RM Lang et al. ACS Pharmacol Transl Sci 2024; Cemdomespib Therapy Slows the Progression of Neuromuscular Weakness and Demyelination in the R75W-Connexin 32 Animal Model of Charcot–Marie–Tooth 1X Disease
Abdel-Halim NHM et al. Cureus 2024; 16 (7) : e65726 Effect of New Antidiabetics on Steatosis in Nerve Tissues and Nerve Conduction Velocity: Possible Role of Nerve Growth Factor (NGF)/Synaptophysin and Nrf2/HO-1 Pathways.
NHM Abdel-Halim et al. Cureus 2024; 16 (7) : e65726 Effect of New Antidiabetics on Steatosis in Nerve Tissues and Nerve Conduction Velocity: Possible Role of Nerve Growth Factor (NGF)/Synaptophysin and Nrf2/HO-1 Pathways
Chen PA et al. Am J Cancer Res 2024; 14 (5) : 2103-2123 The lncRNA TPT1-AS1 promotes the survival of neuroendocrine prostate cancer cells by facilitating autophagy.
X Huang et al. Neuromolecular Med 2024; 26 (1) : 24 FMOD Alleviates Depression-Like Behaviors by Targeting the PI3K/AKT/mTOR Signaling After Traumatic Brain Injury
Show More +
Submit a Reference

REVIEW

Synaptophysin antibody Cat. No. GTX100865

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865
Date : Anonymous submitted on 11-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Brain tissue lysate
Amount used : 20-30 μg
Blocking : 3% milk powder in TBST, 25°C, 1Hr
Primary Antibody : 1:8000, 4°C, 8Hr
Figure Note : Numbers indicated separate mice; L: left and R: right hippocampus and prefrontal cortex (PFC).
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865
Date : Anonymous submitted on 25-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : LNCaP cells
Amount used : 40μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Date : Anonymous submitted on 23-Mar-2012
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Small Intestine tissue
Primary Antibody : 1:200, 4°C, 16Hr
Submit a Review
Application Reference
RM Lang et al. ACS Pharmacol Transl Sci 2024; Cemdomespib Therapy Slows the Progression of Neuromuscular Weakness and Demyelination in the R75W-Connexin 32 Animal Model of Charcot–Marie–Tooth 1X Disease
Abdel-Halim NHM et al. Cureus 2024; 16 (7):e65726 Effect of New Antidiabetics on Steatosis in Nerve Tissues and Nerve Conduction Velocity: Possible Role of Nerve Growth Factor (NGF)/Synaptophysin and Nrf2/HO-1 Pathways.
NHM Abdel-Halim et al. Cureus 2024; 16 (7):e65726 Effect of New Antidiabetics on Steatosis in Nerve Tissues and Nerve Conduction Velocity: Possible Role of Nerve Growth Factor (NGF)/Synaptophysin and Nrf2/HO-1 Pathways
Chen PA et al. Am J Cancer Res 2024; 14 (5):2103-2123 The lncRNA TPT1-AS1 promotes the survival of neuroendocrine prostate cancer cells by facilitating autophagy.
X Huang et al. Neuromolecular Med 2024; 26 (1):24 FMOD Alleviates Depression-Like Behaviors by Targeting the PI3K/AKT/mTOR Signaling After Traumatic Brain Injury
M?dl B et al. EMBO Rep 2023; 24 (10):e57084 Defects in microvillus crosslinking sensitize to colitis and inflammatory bowel disease.
CH Chang et al. Am J Cancer Res 2023; REST-repressed lncRNA LINC01801 induces neuroendocrine differentiation in prostate cancer via transcriptional activation of autophagy
Application : WB
Chiu YS et al. PLoS One 2023; 18 (9):e0291927 Peptide immunization against the C-terminal of alpha-synuclein reduces locomotor activity in mice overexpressing alpha-synuclein.
Du M et al. Mol Psychiatry 2022; 27 (11):4575-4589 A novel circular RNA, circIgfbp2, links neural plasticity and anxiety through targeting mitochondrial dysfunction and oxidative stress-induced synapse dysfunction after traumatic brain injury.
Application : WB, ICC/IF
Reactivity : Human
Hiyori Matsumoto et al. Exp Brain Res 2021; 239 (8):2537-2550 Paradoxical changes in mood-related behaviors on continuous social isolation after weaning.
Application : WB
Reactivity : Rat
Wu Y et al. Diagn Pathol 2021; 16 (1):78 Prognostic significance of the aberrant expression of neuroendocrine markers in melanomas.
Application : IHC-P
Reactivity : Human
Pfl?gler S et al. Commun Biol 2020; 3 (1):252 IDO1+ Paneth cells promote immune escape of colorectal cancer.
Application : IHC-P
Reactivity : Mouse
Pereira JA et al. Hum Mol Genet 2020; (Epub) Mice carrying an analogous heterozygous Dynamin 2 K562E mutation that causes neuropathy in humans develop predominant characteristics of a primary myopathy.
Application : IHC-Wm
Reactivity : Mouse
Bilousova T et al. Am J Pathol 2019; (Epub) Apolipoprotein E/Amyloid-帣 Complex Accumulates in Alzheimer Disease Cortical Synapses via Apolipoprotein E Receptors and Is Enhanced by APOE4.
Application : ICC/IF
Reactivity : Human
Donadon I et al. Nucleic Acids Res 2019; (Epub) Rescue of spinal muscular atrophy mouse models with AAV9-Exon-specific U1 snRNA.
Application : IHC-Fr
Reactivity : Mouse
Crn?ec I et al. Mol Oncol 2018; 12 (4):514-528 STAT1 is a sex-specific tumor suppressor in colitis-associated colorectal cancer.
Zhang L et al. Neurogastroenterol Motil 2018;:e13430 Sustained pain hypersensitivity in the stressed colon: Role of mast cell-derived nerve growth factor-mediated enteric synaptic plasticity.
Application : IHC-P
Reactivity : Rat
Srivatsa S et al. Gastroenterology 2017; 153 (1):178-190.e10 EGFR in Tumor-Associated Myeloid Cells Promotes Development of Colorectal Cancer in Mice and Associates With Outcomes of?Patients.
Pfanzagl B et al. Sci Rep 2017; 7 (1):1313 Activation of the ileal neuroendocrine tumor cell line P-STS by acetylcholine is amplified by histamine: role of H3R and H4R.
Chang YT et al. Sci Rep 2017; 7:42795 REST is a crucial regulator for acquiring EMT-like and stemness phenotypes in hormone-refractory prostate cancer.
Ohta E et al. Hum Mol Genet 2015; 24 (17):4879-900 I2020T mutant LRRK2 iPSC-derived neurons in the Sagamihara family exhibit increased Tau phosphorylation through the AKT/GSK-3β signaling pathway.
Application : WB
Reactivity : Human
Thomas MG et al. Cell Death Dis 2013; 4:e669 Nicotinamide N-methyltransferase expression in SH-SY5Y neuroblastoma and N27 mesencephalic neurones induces changes in cell morphology via ephrin-B2 and Akt signalling.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 419
$ 169