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Synaptophysin antibody

Orthogonal
Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in IHC (Frozen sections) (IHC-Fr). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

Cat No. GTX100865

Reference 12
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Synaptophysin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

human brain , mouse brain , rat brain , *Mouse small intestine , *LNCaP

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Synaptophysin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038077

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

synaptophysin , MRX96 , MRXSYP

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Multi-pass membrane protein , Cell junction , synapse , synaptosome

Background

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865

GTX100865 ICC/IF Image

Synaptophysin antibody detects Synaptophysin protein at synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptophysin protein stained by Synaptophysin antibody (GTX100865) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Orthogonal

Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.
Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4ºC and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody used in IHC (Frozen sections) (IHC-Fr). GTX100865

GTX100865 IHC-Fr Image

Synaptophysin antibody detects Synaptophysin protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse muscle.
Green: Synaptophysin stained by Synaptophysin antibody (GTX100865) diluted at 1:250.
Red: α-Bungarotoxin, stained by α-Bungarotoxin, Alexa Fluor™ 594 conjugate (B13423) diluted at 1:5000.
Blue: Hoechst 33342 staining.

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: Synaptophysin protein stained by Synaptophysin antibody (GTX100865) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100865

GTX100865 ICC/IF Image

Synaptophysin antibody detects Synaptophysin protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptophysin stained by Synaptophysin antibody (GTX100865) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865

GTX100865 WB Image

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody (GTX100865) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865

GTX100865 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody (GTX100865) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Synaptophysin stained by Synaptophysin antibody (GTX100865) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Synaptophysin stained by Synaptophysin antibody (GTX100865) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Synaptophysin antibody detects Synaptophysin protein at on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Synaptophysin antibody (GTX100865) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865

GTX100865 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft , using Synaptophysin(GTX100865) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Pfl?gler S et al. Commun Biol 2020; 3 (1) : 252 IDO1+ Paneth cells promote immune escape of colorectal cancer.
Reactivity : Mouse
Pfl?gler S et al. Commun Biol 2020; 3 (1) : 252 IDO1+ Paneth cells promote immune escape of colorectal cancer.
Application : IHC-P
Pereira JA et al. Hum Mol Genet 2020; (Epub) Mice carrying an analogous heterozygous Dynamin 2 K562E mutation that causes neuropathy in humans develop predominant characteristics of a primary myopathy.
Application : IHC-Wm
Reactivity : Mouse
Bilousova T et al. Am J Pathol 2019; (Epub) Apolipoprotein E/Amyloid-帣 Complex Accumulates in Alzheimer Disease Cortical Synapses via Apolipoprotein E Receptors and Is Enhanced by APOE4.
Application : ICC/IF
Reactivity : Human
Donadon I et al. Nucleic Acids Res 2019; (Epub) Rescue of spinal muscular atrophy mouse models with AAV9-Exon-specific U1 snRNA.
Application : IHC-Fr
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

Synaptophysin antibody Cat No. GTX100865

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-Synaptophysin antibody used in Western Blot (WB). GTX100865
Date : Anonymous submitted on 25-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : LNCaP cells
Amount used : 40μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Synaptophysin antibody used in IHC (Paraffin sections) (IHC-P). GTX100865
Date : Anonymous submitted on 23-Mar-2012
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Small Intestine tissue
Primary Antibody : 1:200, 4°C, 16Hr
Submit a Review
Application Reference
Pfl?gler S et al. Commun Biol 2020; 3 (1):252 IDO1+ Paneth cells promote immune escape of colorectal cancer.
Reactivity : Mouse
Pfl?gler S et al. Commun Biol 2020; 3 (1):252 IDO1+ Paneth cells promote immune escape of colorectal cancer.
Application : IHC-P
Pereira JA et al. Hum Mol Genet 2020; (Epub) Mice carrying an analogous heterozygous Dynamin 2 K562E mutation that causes neuropathy in humans develop predominant characteristics of a primary myopathy.
Application : IHC-Wm
Reactivity : Mouse
Bilousova T et al. Am J Pathol 2019; (Epub) Apolipoprotein E/Amyloid-帣 Complex Accumulates in Alzheimer Disease Cortical Synapses via Apolipoprotein E Receptors and Is Enhanced by APOE4.
Application : ICC/IF
Reactivity : Human
Donadon I et al. Nucleic Acids Res 2019; (Epub) Rescue of spinal muscular atrophy mouse models with AAV9-Exon-specific U1 snRNA.
Application : IHC-Fr
Reactivity : Mouse
Crn?ec I et al. Mol Oncol 2018; 12 (4):514-528 STAT1 is a sex-specific tumor suppressor in colitis-associated colorectal cancer.
Zhang L et al. Neurogastroenterol Motil 2018;:e13430 Sustained pain hypersensitivity in the stressed colon: Role of mast cell-derived nerve growth factor-mediated enteric synaptic plasticity.
Application : IHC-P
Reactivity : Rat
Srivatsa S et al. Gastroenterology 2017; 153 (1):178-190.e10 EGFR in Tumor-Associated Myeloid Cells Promotes Development of Colorectal Cancer in Mice and Associates With Outcomes of?Patients.
Pfanzagl B et al. Sci Rep 2017; 7 (1):1313 Activation of the ileal neuroendocrine tumor cell line P-STS by acetylcholine is amplified by histamine: role of H3R and H4R.
Chang YT et al. Sci Rep 2017; 7:42795 REST is a crucial regulator for acquiring EMT-like and stemness phenotypes in hormone-refractory prostate cancer.
Ohta E et al. Hum Mol Genet 2015; 24 (17):4879-900 I2020T mutant LRRK2 iPSC-derived neurons in the Sagamihara family exhibit increased Tau phosphorylation through the AKT/GSK-3β signaling pathway.
Application : WB
Reactivity : Human
Thomas MG et al. Cell Death Dis 2013; 4:e669 Nicotinamide N-methyltransferase expression in SH-SY5Y neuroblastoma and N27 mesencephalic neurones induces changes in cell morphology via ephrin-B2 and Akt signalling.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169