GeneTex
United States (US)

Synaptophysin antibody

Synaptophysin-antibody-GTX14692-WB-1_18121411_196.jpg
Synaptophysin-antibody_WB_GTX14692-1_18121411_682.jpg

Cat No. GTX14692

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, IHC
Reactivity Human, Mouse, Rat, Bovine
APPLICATION

Application Note

IHC: Use at an assay dependent dilution. IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/1000. Predicted molecular weight: 34 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

34 kDa. ( Note )

Positive Control

Rat brain and pancreatic homogenates

Specificity/Sensitivity

The antibody does not cross-react other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens.
PROPERTIES

Form

Liquid

Buffer

Tris/glycine buffer at pH 7.4, containing 0.02% sodium azide as preservative, cryo-protective agents, stabilizing proteins (BSA) and 30% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: FATCGSYSGELQLSVDCANKTE, corresponding to amino acids 41-62 of Human Synaptophysin.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Synaptophysin,Mrx96,Mrxsyp,Syp

Cellular Localization

Integral membrane protein. Synaptic vesicles.

Background

This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES
Synaptophysin-antibody-GTX14692-WB-1_18121411_196.jpg

GTX14692 WB Image

Synaptophysin antibody(GTX14692) western blot on mouse forebrainextract (10g protein/lane). The blot was exposed for 10minutes to autoradiographic film. Ladder markers from 7-194 kDa. Lane 1 = 1/500 GTX14692, Lane 2 = 1/200 GTX14692

Synaptophysin-antibody_WB_GTX14692-1_18121411_682.jpg

GTX14692 WB Image

Western blot using PC-Syn and 1:1500 diluted GTX14692. Apprximate MW of Synaptophysin is 39kDa

Package List Price ($)
$ 329