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Synaptopodin antibody

Anti-Synaptopodin antibody used in Western Blot (WB). GTX85140
Anti-Synaptopodin antibody used in IHC (Paraffin sections) (IHC-P). GTX85140
Anti-Synaptopodin antibody used in IHC (Paraffin sections) (IHC-P). GTX85140
Anti-Synaptopodin antibody used in IHC (Paraffin sections) (IHC-P). GTX85140
Anti-Synaptopodin antibody used in IHC (Paraffin sections) (IHC-P). GTX85140

Cat. No. GTX85140

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Synaptopodin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Rat Brain Tissue Lysate(GTX27942)

Product Note

At least three isoforms of SYNPO are known to exist; this antibody will detect all three isoforms. SYNPO antibody is predicted to not cross-react with other SYNPO family member proteins.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synaptopodin antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human Synaptopodin.The immunogen is located within the last 50 amino acids of Synaptopodin.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726377

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptopodin

Cellular Localization

Cytoplasm, cytoskeleton,Cell junction, synapse

Background

SYNPO, also known as synaptopodin, is an actin-associated protein in telencephalic dendrites and renal podocytes. SYNPO is tightly associated with the dendritic spine apparatus, and mice lacking the SYNPO gene lack the apparatus. These SYNPO- mice also demonstrate an impaired ability to express long-term potentiation as well as deficits in spatial memory tasks, indicating that SYNPO is involved in the regulation of synaptic plasticity. Recent studies suggest that SYNPO is linked to neuronal calcium stores and plays a role in the calcium store-associated ability of neurons to undergo long-term plasticity.

Database

Research Area

DATA IMAGES

Anti-Synaptopodin antibody used in Western Blot (WB). GTX85140

GTX85140 WB Image

WB analysis of rat brain tissue lysate using GTX85140 Synaptopodin antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-Synaptopodin antibody used in IHC (Paraffin sections) (IHC-P). GTX85140

GTX85140 IHC-P Image

IHC-P analysis of human brain tissue using GTX85140 Synaptopodin antibody.
Working concentration : 2.5 μg/ml

Anti-Synaptopodin antibody used in IHC (Paraffin sections) (IHC-P). GTX85140

GTX85140 IHC-P Image

IHC-P analysis of human brain tissue using GTX85140 Synaptopodin antibody.
Working concentration : 20 μg/ml

Anti-Synaptopodin antibody used in IHC (Paraffin sections) (IHC-P). GTX85140

GTX85140 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85140 Synaptopodin antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml

Anti-Synaptopodin antibody used in IHC (Paraffin sections) (IHC-P). GTX85140

GTX85140 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85140 Synaptopodin antibody.
Dilution : 5 μg/ml

REFERENCE

Application Reference

Abkhezr M et al. Mol Pharmacol 2015; 87 (2) : 231-9 STAT3 regulates steady-state expression of synaptopodin in cultured mouse podocytes.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399