APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for SYT1 protein, and it does not cross-react with SYT2 protein.
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Synaptotagmin 1
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
synaptotagmin 1 , BAGOS , P65 , SVP65 , SYT
Cellular Localization
Cytoplasm
Background
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]
Database
Research Area
DATA IMAGES
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GTX637119 WB Image
Human tissue extract (5 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (GTX637119) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637119 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (GTX637119) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637119 WB Image
Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (GTX637119) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637119 ICC/IF Image
Synaptotagmin 1 antibody [HL1626] detects Synaptotagmin 1 protein at cytoplasm by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1626] (GTX637119) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637119 ICC/IF Image
Synaptotagmin 1 antibody [HL1626] detects Synaptotagmin 1 protein by immunofluorescent analysis.Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1626] (GTX637119) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637119 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (GTX637119) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
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REVIEW
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