Synaptotagmin 1 antibody [HL1626]

Synaptotagmin 1 antibody [HL1626] detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1626] (GTX637119) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (GTX637119) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (GTX637119) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human tissue extract (5 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (GTX637119) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (GTX637119) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.