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Synaptotagmin 1 antibody [HL1654]

Recombinant antibody Orthogonal Overexpression
Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
Anti-Synaptotagmin 1 antibody [HL1654] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637252
Anti-Synaptotagmin 1 antibody [HL1654] used in IHC (Paraffin sections) (IHC-P). GTX637252
Anti-Synaptotagmin 1 antibody [HL1654] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637252
Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252

Cat. No. GTX637252

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1654

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all Synaptotagmin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Product Note

This antibody is specific for SYT1 protein, and it does not cross-react with SYT2 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Synaptotagmin 1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptotagmin 1 , BAGOS , P65 , SVP65 , SYT

Cellular Localization

Cytoplasm

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252

GTX637252 WB Image

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252

GTX637252 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252

GTX637252 WB Image

Human tissue extract (5 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252

GTX637252 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Synaptotagmin 1 antibody [HL1654] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637252

GTX637252 ICC/IF Image

Synaptotagmin 1 antibody [HL1654] detects Synaptotagmin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Synaptotagmin 1 antibody [HL1654] used in IHC (Paraffin sections) (IHC-P). GTX637252

GTX637252 IHC-P Image

Synaptotagmin 1 antibody [HL1654] detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptotagmin 1 antibody [HL1654] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637252

GTX637252 ICC/IF Image

Synaptotagmin 1 antibody [HL1654] detects Synaptotagmin 1 protein by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252

GTX637252 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Package List Price ($)
$ 399
$ 169
Custom Conjugations