GeneTex
United States (US)

Synaptotagmin 1 antibody, Internal

GTX88461_2074_WB_18121411_290.jpg
GTX88461_836_IHC-P_18121411_892.jpg

Cat No. GTX88461

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 4-6μg/ml

Note :

Human Brain shows vesicular staining in neuronal cell and axon.
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88461-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-RDLQSAEKEEQEK, from the internal region of the protein sequence according to NP_005630.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P65 Antibody , SVP65 Antibody , SYT Antibody , SYT1 Antibody

Cellular Localization

Synaptic vesicles and chromaffin granules.

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]
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Database

Research Area

DATA IMAGES
GTX88461_2074_WB_18121411_290.jpg

GTX88461 WB Image

WB analysis of human hippocampus lysate using GTX88461 Synaptotagmin 1 antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

GTX88461_836_IHC-P_18121411_892.jpg

GTX88461 IHC-P Image

IHC-P analysis of human brain using GTX88461 Synaptotagmin 1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml