GeneTex
  • Country / Location Selection

United States (US)

Synaptotagmin 1 antibody [N1C2]

Anti-Synaptotagmin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114124
Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124
Anti-Synaptotagmin 1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114124
Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124

Cat No. GTX114124

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Synaptotagmin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163039

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

synaptotagmin 1 , BAGOS , P65 , SVP65 , SYT

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Single-pass membrane protein , chromaffin granule membrane; Single-pass membrane protein , Cytoplasm

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Synaptotagmin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114124

GTX114124 IHC-P Image

Synaptotagmin 1 antibody [N1C2] detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Synaptotagmin 1 stained by Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124

GTX114124 WB Image

Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptotagmin 1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114124

GTX114124 ICC/IF Image

Synaptotagmin 1 antibody [N1C2] detects Synaptotagmin 1 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptotagmin 1 protein stained by Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124

GTX114124 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

There are currently no references for Synaptotagmin 1 antibody [N1C2] (GTX114124). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Synaptotagmin 1 antibody [N1C2] (GTX114124). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169