APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , rat brain
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Synaptotagmin 1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11163039
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
synaptotagmin 1 , BAGOS , P65 , SVP65 , SYT
Cellular Localization
Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Single-pass membrane protein , chromaffin granule membrane; Single-pass membrane protein , Cytoplasm
Background
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-Synaptotagmin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114124](/templates/default/js/lightbox/images/loading.gif "Anti-Synaptotagmin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114124")
|
GTX114124 IHC-P Image
Synaptotagmin 1 antibody [N1C2] detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: Synaptotagmin 1 stained by Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX114124 WB Image
Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX114124 ICC/IF Image
Synaptotagmin 1 antibody [N1C2] detects Synaptotagmin 1 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptotagmin 1 protein stained by Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
|
|
|
GTX114124 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
REFERENCE
There are currently no references for Synaptotagmin 1 antibody [N1C2] (GTX114124). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Synaptotagmin 1 antibody [N1C2] (GTX114124). Be the first to share your experience with this product.
![Anti-Synaptotagmin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114124](/upload/website/prouct_img/small/GTX114124/GTX114124_40583_20201225_IHC-P_M_21011401_517.jpg "Anti-Synaptotagmin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114124")
![Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124](/upload/website/prouct_img/small/GTX114124/GTX114124_40583_20170728_WB_M_R_18121410_281.jpg "Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124")
![Anti-Synaptotagmin 1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114124](/upload/website/prouct_img/small/GTX114124/GTX114124_40583_20170719_IFA_R_18121410_785.jpg "Anti-Synaptotagmin 1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114124")
![Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124](/upload/website/prouct_img/small/GTX114124/GTX114124_40583_20211203_WB_21120523_644.jpg "Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124")
![Anti-Synaptotagmin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114124](/upload/website/prouct_img/normal/GTX114124/GTX114124_40583_20201225_IHC-P_M_21011401_517.jpg "Anti-Synaptotagmin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114124")
![Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124](/upload/website/prouct_img/normal/GTX114124/GTX114124_40583_20170728_WB_M_R_18121410_281.jpg "Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124")
![Anti-Synaptotagmin 1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114124](/upload/website/prouct_img/normal/GTX114124/GTX114124_40583_20170719_IFA_R_18121410_785.jpg "Anti-Synaptotagmin 1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114124")
![Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124](/upload/website/prouct_img/normal/GTX114124/GTX114124_40583_20211203_WB_21120523_644.jpg "Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124")