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Synaptotagmin 1 antibody [N1C2]

Orthogonal Overexpression
Anti-Synaptotagmin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114124
Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124
Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124
Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124
Anti-Synaptotagmin 1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114124
Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124
Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124

Cat. No. GTX114124

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Synaptotagmin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Synaptotagmin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163039

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptotagmin 1 , BAGOS , P65 , SVP65 , SYT

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Single-pass membrane protein , chromaffin granule membrane; Single-pass membrane protein , Cytoplasm

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Synaptotagmin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114124

GTX114124 IHC-P Image

Synaptotagmin 1 antibody [N1C2] detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Synaptotagmin 1 stained by Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124

GTX114124 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124

GTX114124 WB Image

Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124

GTX114124 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Synaptotagmin 1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114124

GTX114124 ICC/IF Image

Synaptotagmin 1 antibody [N1C2] detects Synaptotagmin 1 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptotagmin 1 protein stained by Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124

GTX114124 WB Image

Orthogonal Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptotagmin 1 antibody [N1C2] used in Western Blot (WB). GTX114124

GTX114124 WB Image

Orthogonal

Various whole cell extracts (30 Âμg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Hong CT et al. Elife 2024; 12 Plasma extracellular vesicle synaptic proteins as biomarkers of clinical progression in patients with Parkinson's disease.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169