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Synaptotagmin 1 antibody [N3C3]

Anti-Synaptotagmin 1 antibody [N3C3] used in Western Blot (WB). GTX103641
Anti-Synaptotagmin 1 antibody [N3C3] used in Western Blot (WB). GTX103641
Anti-Synaptotagmin 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX103641

Cat No. GTX103641

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Synaptotagmin 1 tansfected 293T cell , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Synaptotagmin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

synaptotagmin 1 , BAGOS , P65 , SVP65 , SYT

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Single-pass membrane protein , chromaffin granule membrane; Single-pass membrane protein , Cytoplasm

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Synaptotagmin 1 antibody [N3C3] used in Western Blot (WB). GTX103641

GTX103641 WB Image

Western Blot analysis of SYT1 expression in transfected 293T cell line by SYT1 polyclonal antibody.
A: Non-transfected lysate.
B: SYT1 transfected lysate.
7.5% SDS PAGE
GTX103641 diluted at 1:500

Anti-Synaptotagmin 1 antibody [N3C3] used in Western Blot (WB). GTX103641

GTX103641 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [N3C3] (GTX103641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synaptotagmin 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX103641

GTX103641 IHC-P Image

Synaptotagmin 1 antibody [N3C3] detects Synaptotagmin 1 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Synaptotagmin 1 antibody [N3C3] (GTX103641) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169