GeneTex
United States (US)

Synaptotagmin 1 antibody [N3C3]

GTX103641_WB_19011506_484.jpg
GTX103641_39883_20170728_WB_M_R_19011506_986.jpg
GTX103641_39883_IHC_19011506_216.jpg

Cat No. GTX103641

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Synaptotagmin 1 tansfected 293T cell , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Synaptotagmin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Synaptotagmin 1,P65,Svp65,Syt,Syt1

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Single-pass membrane protein , chromaffin granule membrane; Single-pass membrane protein , Cytoplasm

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX103641_WB_19011506_484.jpg

GTX103641 WB Image

Western Blot analysis of SYT1 expression in transfected 293T cell line by SYT1 polyclonal antibody.
A: Non-transfected lysate.
B: SYT1 transfected lysate.
7.5% SDS PAGE
GTX103641 diluted at 1:500

GTX103641_39883_20170728_WB_M_R_19011506_986.jpg

GTX103641 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [N3C3] (GTX103641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103641_39883_IHC_19011506_216.jpg

GTX103641 IHC-P Image

Synaptotagmin 1 antibody [N3C3] detects Synaptotagmin 1 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Synaptotagmin 1 antibody [N3C3] (GTX103641) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169