*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Synaptotagmin 1 tansfected 293T cell , mouse brain , rat brain
Bovine, Chicken(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.75 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human Synaptotagmin 1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Single-pass membrane protein , chromaffin granule membrane; Single-pass membrane protein , Cytoplasm
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM]
GTX103641 WB Image
Western Blot analysis of SYT1 expression in transfected 293T cell line by SYT1 polyclonal antibody.
A: Non-transfected lysate.
B: SYT1 transfected lysate.
7.5% SDS PAGE
GTX103641 diluted at 1:500
GTX103641 WB Image
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [N3C3] (GTX103641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX103641 IHC-P Image
Synaptotagmin 1 antibody [N3C3] detects Synaptotagmin 1 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Synaptotagmin 1 antibody [N3C3] (GTX103641) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min