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Synaptotagmin 1 antibody

Anti-Synaptotagmin 1 antibody used in Immunohistochemistry (IHC). GTX13260
Anti-Synaptotagmin 1 antibody used in Western Blot (WB). GTX13260

Cat No. GTX13260

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, IHC
Reactivity Human, Mouse, Rat, Bovine, Xenopus laevis
Package
50 μl ($319)
APPLICATION

Application Note

ICC: Use at an assay dependent dilution. IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/1000. This dilution was sufficient for detection of Synaptotagmin in 20 μg of rat brain tissue extract. Detects a band of approximately 65 kDa (predicted molecular weight: 50.5 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

48 kDa. ( Note )

Positive Control

Rat brain tissue extract
PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: Whole Serum

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Synthetic peptide: MVSASHPEALAAPVTTVATLVPH(C) conjugated to KLH, corresponding to amino acids 1-23 of Rat Synaptotagmin I.

Purification

Whole antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Synaptotagmin 1,P65,Svp65,Syt,Syt1

Cellular Localization

Synaptic vesicles and chromaffin granules.

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]

Database

Research Area

DATA IMAGES
Anti-Synaptotagmin 1 antibody used in Immunohistochemistry (IHC). GTX13260

GTX13260 IHC Image

Immunohistochemistry analysis of human colon tissue stained with Synaptotagmin I, pAb at 20µg/ml.

Anti-Synaptotagmin 1 antibody used in Western Blot (WB). GTX13260

GTX13260 WB Image

Western blot analysis:
Lane 1: Rat Brain
Lane 2: Mouse Brain
Lane 3: Xenopus Ovary, probed with Synaptotagmin I pAb (GTX13260)

REFERENCE
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Package List Price ($)
$ 319