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Synaptotagmin 1 (phospho Ser312) + Synaptotagmin 2 (phospho Ser309) antibody

Anti-Synaptotagmin 1 (phospho Ser312) + Synaptotagmin 2 (phospho Ser309) antibody used in Western Blot (WB). GTX82583
Anti-Synaptotagmin 1 (phospho Ser312) + Synaptotagmin 2 (phospho Ser309) antibody used in Immunohistochemistry (IHC). GTX82583

Cat. No. GTX82583

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC

Reactivity

 Rat
Package
100 μl ($499)
See all Synaptotagmin 1 + Synaptotagmin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Product Note

The sequence around Synaptotagmin 1 (phospho Ser312) is conserved with the sequence around Synaptotagmin 2 (phospho Ser309).

PROPERTIES

Form

Liquid

Buffer

10mM HEPES, 150mM NaCl, 0.01% BSA, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 conjugated to KLH.

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptotagmin 1 , P65

Cellular Localization

Cytoplasm

Background

integral component of synaptic vesicle membranes; important for exocytosis and vesicle trafficking to the active zone of synapses; may be the calcium sensor for calcium-dependent exocytosis [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Synaptotagmin 1 (phospho Ser312) + Synaptotagmin 2 (phospho Ser309) antibody used in Western Blot (WB). GTX82583

GTX82583 WB Image

Western blot of rat cortex lysate showing specific immunolabeling of the ~62k synaptotagmin phosphorylated at Ser309 (Control). Phosphospecificity is shown in the second lane (lambdaphosphatase: λ-Ptase).

Anti-Synaptotagmin 1 (phospho Ser312) + Synaptotagmin 2 (phospho Ser309) antibody used in Immunohistochemistry (IHC). GTX82583

GTX82583 IHC Image

Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green using Synaptotagmin 1 (phospho Ser312) + Synaptotagmin 2 (phospho Ser309) antibody (GTX82583).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 499