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Synaptotagmin I/II antibody

Anti-Synaptotagmin I/II antibody used in Western Blot (WB). GTX78914

Cat No. GTX78914

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human Synaptotagmin I/II around the phosphorylation site of threonine 202/199 (R-K-Tp-L-N).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_11173744

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

P65,SVP65,SYT,SYT1,synaptotagmin 1,Synaptotagmin 1

Cellular Localization

Cytoplasmic vesicle,Secretory vesicle membrane,Secretory vesicle,Synaptic vesicle membrane,Chromaffin granule membrane,Cytoplasm

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Synaptotagmin I/II antibody used in Western Blot (WB). GTX78914

GTX78914 WB Image

WB analysis of HeLa cell lysate using GTX78914 Synaptotagmin I/II antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 379