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Synaptotagmin I/II antibody

Anti-Synaptotagmin I/II antibody used in Western Blot (WB). GTX78914

Cat. No. GTX78914

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all Synaptotagmin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human Synaptotagmin I/II around the phosphorylation site of threonine 202/199 (R-K-Tp-L-N).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_11173744

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

P65,SVP65,SYT,SYT1,synaptotagmin 1,Synaptotagmin 1

Cellular Localization

Cytoplasmic vesicle,Secretory vesicle membrane,Secretory vesicle,Synaptic vesicle membrane,Chromaffin granule membrane,Cytoplasm

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Synaptotagmin I/II antibody used in Western Blot (WB). GTX78914

GTX78914 WB Image

WB analysis of HeLa cell lysate using GTX78914 Synaptotagmin I/II antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399