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Synaptotagmin antibody [ASV48]

Anti-Synaptotagmin antibody [ASV48] used in Western Blot (WB). GTX12255

Cat No. GTX12255

Host

Mouse

Clonality

Monoclonal

Clone Name

ASV48

Isotype

IgG2b

Application

WB, ICC/IF, IP, IHC

Reactivity

Mouse, Rat, Bovine, Monkey, Shark
Package
100 μg ($379)

APPLICATION

Application Note

ICC: Use at a dilution of 1/500. IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.1 μg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 50.5 kDa). Optimal dilutions/concentrations should be determined by the end user.

Positive Control

Rat brain tissue extract.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1mM PMSF pH7.2, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Antigen: Rat brain synaptic junction protein complexes.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SVP65 Antibody , SYT Antibody , P65 Antibody , SYT1 Antibody

Cellular Localization

Synaptic vesicles and chromaffin granules.

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]

Research Area

DATA IMAGES

Anti-Synaptotagmin antibody [ASV48] used in Western Blot (WB). GTX12255

GTX12255 WB Image

Western blot analysis of rat brain tissue extract, probed with Synaptotagmin, mAb (ASV48).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379