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Syndecan-1 / CD138 antibody [1A3H4]

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Flow cytometry (FACS). GTX00451
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00451
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Western Blot (WB). GTX00451
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in IHC (Paraffin sections) (IHC-P). GTX00451
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in ELISA (ELISA). GTX00451
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in IHC (Paraffin sections) (IHC-P). GTX00451
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Western Blot (WB). GTX00451

Cat No. GTX00451

Host Mouse
Clonality Monoclonal
Clone Name 1A3H4
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity Human, Mouse
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200-1:1000
ICC/IF 1:200-1:1000
IHC-P 1:200-1:1000
FACS 1:200-1:400
ELISA 1:10000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A recombinant fragment of human SDC1 (AA: 28-171) expressed in E. coli.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

syndecan 1,CD138,SDC,SYND1,syndecan

Cellular Localization

Membrane,Secreted

Background

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Flow cytometry (FACS). GTX00451

GTX00451 FACS Image

FACS analysis of HepG2 cells using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Green : Primary antibody
Red : Negative control (without primary antibody)

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00451

GTX00451 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Green : Primary antibody
Blue: DRAQ5 fluorescent DNA dye

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Western Blot (WB). GTX00451

GTX00451 WB Image

WB analysis of various samples using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Lane 1 : HEK293 cell lysate
Land 2 : SDC1 (AA: 28-171)-hIgGFc transfected HEK293 cell lysate

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in IHC (Paraffin sections) (IHC-P). GTX00451

GTX00451 IHC-P Image

IHC-P analysis of rectum cancer tissues using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in ELISA (ELISA). GTX00451

GTX00451 ELISA Image

ELISA analysis of antigen using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in IHC (Paraffin sections) (IHC-P). GTX00451

GTX00451 IHC-P Image

IHC-P analysis of ovarian cancer tissues using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Western Blot (WB). GTX00451

GTX00451 WB Image

WB analysis of various samples using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Lane 1 : MCF-7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : T47D cell lysate
Lane 5 : SW620 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : NIH/3T3 cell lysate

REFERENCE
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REVIEW
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Package List Price ($)
$ 319