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Syndecan-1 / CD138 antibody [1A3H4]

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Flow cytometry (FACS). GTX00451
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00451
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Western Blot (WB). GTX00451
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in IHC (Paraffin sections) (IHC-P). GTX00451
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in ELISA (ELISA). GTX00451
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in IHC (Paraffin sections) (IHC-P). GTX00451
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Western Blot (WB). GTX00451

Cat No. GTX00451

Host Mouse
Clonality Monoclonal
Clone Name 1A3H4
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity Human, Mouse
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200-1:1000
ICC/IF 1:200-1:1000
IHC-P 1:200-1:1000
FACS 1:200-1:400
ELISA 1:10000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A recombinant fragment of human SDC1 (AA: 28-171) expressed in E. coli.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

syndecan 1 , CD138 , SDC , SYND1 , syndecan

Cellular Localization

Membrane,Secreted

Background

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Flow cytometry (FACS). GTX00451

GTX00451 FACS Image

FACS analysis of HepG2 cells using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Green : Primary antibody
Red : Negative control (without primary antibody)

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00451

GTX00451 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Green : Primary antibody
Blue: DRAQ5 fluorescent DNA dye

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Western Blot (WB). GTX00451

GTX00451 WB Image

WB analysis of various samples using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Lane 1 : HEK293 cell lysate
Land 2 : SDC1 (AA: 28-171)-hIgGFc transfected HEK293 cell lysate

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in IHC (Paraffin sections) (IHC-P). GTX00451

GTX00451 IHC-P Image

IHC-P analysis of rectum cancer tissues using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in ELISA (ELISA). GTX00451

GTX00451 ELISA Image

ELISA analysis of antigen using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in IHC (Paraffin sections) (IHC-P). GTX00451

GTX00451 IHC-P Image

IHC-P analysis of ovarian cancer tissues using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].

Anti-Syndecan-1 / CD138 antibody [1A3H4] used in Western Blot (WB). GTX00451

GTX00451 WB Image

WB analysis of various samples using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Lane 1 : MCF-7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : T47D cell lysate
Lane 5 : SW620 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : NIH/3T3 cell lysate

REFERENCE
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REVIEW
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Package List Price ($)
$ 319