Syndecan-1 / CD138 antibody [1A3H4]

Cat. No. GTX00451

Host	Mouse
Clonality	Monoclonal
Clone Name	1A3H4
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse
Package	100 μl ($419)

See all Syndecan-1 / CD138 products

Datasheet
Document

View product citations for antibody GTX00451 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:200-1:1000
ICC/IF	1:200-1:1000
IHC-P	1:200-1:1000
FACS	1:200-1:400
ELISA	1:10000

Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A recombinant fragment of human SDC1 (AA: 28-171) expressed in E. coli.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

syndecan 1 , CD138 , SDC , SYND1 , syndecan

Cellular Localization

Membrane,Secreted

Background

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

Database

Gene ID: 6382 SDC1
UniProt: P18827 SDC1

Research Area

DATA IMAGES

GTX00451 ELISA Image

ELISA analysis of antigen using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX00451 WB Image

WB analysis of various samples using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Lane 1 : MCF-7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : T47D cell lysate
Lane 5 : SW620 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : NIH/3T3 cell lysate

GTX00451 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Green : Primary antibody
Blue: DRAQ5 fluorescent DNA dye

GTX00451 WB Image

WB analysis of various samples using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Lane 1 : HEK293 cell lysate
Land 2 : SDC1 (AA: 28-171)-hIgGFc transfected HEK293 cell lysate

GTX00451 FACS Image

FACS analysis of HepG2 cells using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Green : Primary antibody
Red : Negative control (without primary antibody)

GTX00451 IHC-P Image

IHC-P analysis of ovarian cancer tissues using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].

GTX00451 IHC-P Image

IHC-P analysis of rectum cancer tissues using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 419

Qty

Syndecan-1 / CD138 antibody [1A3H4]

Cat. No. GTX00451

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX00451 ELISA Image

GTX00451 WB Image

GTX00451 ICC/IF Image

GTX00451 WB Image

GTX00451 FACS Image

GTX00451 IHC-P Image

GTX00451 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.