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Syntaxin 4 antibody [N2C3]

KO/KD
Anti-Syntaxin 4 antibody [N2C3] used in Western Blot (WB). GTX114806
Anti-Syntaxin 4 antibody [N2C3] used in Western Blot (WB). GTX114806

Cat. No. GTX114806

Reference 1
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Syntaxin 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

A431 , K562 , THP-1 , HL-60

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Syntaxin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624584

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

syntaxin 4 , STX4A , p35-2

Cellular Localization

Cell membrane; Single-pass type IV membrane protein

Background

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.

Database

Research Area

DATA IMAGES

Anti-Syntaxin 4 antibody [N2C3] used in Western Blot (WB). GTX114806

GTX114806 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX114806 diluted at 1:3000

Anti-Syntaxin 4 antibody [N2C3] used in Western Blot (WB). GTX114806

GTX114806 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Syntaxin 4 antibody [N2C3] (GTX114806) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Kuge H et al. J Lipid Res 2020; 61 (12) : 1747-1763 PLRP2 selectively localizes synaptic membrane proteins via acyl-chain remodeling of phospholipids.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

Syntaxin 4 antibody [N2C3] Cat. No. GTX114806

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Syntaxin 4 antibody [N2C3] used in Immunoprecipitation (IP). GTX114806
Date : Anonymous submitted on 26-Jul-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 cells
Lane Description :
SM: 10% starting material
UB: 10% unbound fraction
IP: IP antibody
Figure Note :
2.0μg of antibody to 500μl of Pierce IP Lysis Buffer, together with 30μl of protein A- Sepharose beads.
One ml aliquots at 0.5 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Syntaxin 4 antibody [N2C3] used in Western Blot (WB). GTX114806
Date : Anonymous submitted on 26-Jul-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and STX4 KO cells
Amount used : 60μg
Blocking : 5% milk, 1 hr
Primary Antibody : 1:1000 in 5% BSA and TBST, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169