GeneTex
United States (US)

Syntaxin 5 antibody

GTX114807_40233_WB_R_18121410_390.jpg
GTX114807_40233_20171115_ICC_IF_R_18121410_974.jpg
GTX114807_40233_IHC_18121410_950.jpg
GTX114807_WB_18121410_339.jpg

Cat No. GTX114807

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

A431 , PC-12

Predict Reactivity

Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Syntaxin 5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Syntaxin 5,Sed5,Stx5A,Stx5

Cellular Localization

Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein , Golgi apparatus membrane; Single-pass type IV membrane protein

Background

Mediates endoplasmic reticulum to Golgi transport.

Database

DATA IMAGES
GTX114807_40233_WB_R_18121410_390.jpg

GTX114807 WB Image

Sample (30 µg of whole cell lysate)
A: PC-12
12% SDS PAGE
GTX114807 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114807_40233_20171115_ICC_IF_R_18121410_974.jpg

GTX114807 ICC/IF Image

Syntaxin 5 antibody detects Syntaxin 5 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Syntaxin 5 stained by Syntaxin 5 antibody (GTX114807) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX114807_40233_IHC_18121410_950.jpg

GTX114807 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Syntaxin 5(GTX114807) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114807_WB_18121410_339.jpg

GTX114807 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX114807 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169