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Syntaxin 6 antibody [N1C3]

Anti-Syntaxin 6 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115375
Anti-Syntaxin 6 antibody [N1C3] used in Western Blot (WB). GTX115375
Anti-Syntaxin 6 antibody [N1C3] used in Western Blot (WB). GTX115375
Anti-Syntaxin 6 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115375

Cat No. GTX115375

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Syntaxin 6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621410

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

syntaxin 6

Cellular Localization

Golgi apparatus membrane; Single-pass type IV membrane protein

Background

Involved in intracellular vesicle trafficking.

Database

Research Area

DATA IMAGES

Anti-Syntaxin 6 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115375

GTX115375 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Syntaxin 6(GTX115375) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Syntaxin 6 antibody [N1C3] used in Western Blot (WB). GTX115375

GTX115375 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX115375 diluted at 1:1000

Anti-Syntaxin 6 antibody [N1C3] used in Western Blot (WB). GTX115375

GTX115375 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntaxin 6 antibody [N1C3] (GTX115375) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Syntaxin 6 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115375

GTX115375 ICC/IF Image

Syntaxin 6 antibody [N1C3] detects Syntaxin 6 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Syntaxin 6 protein stained by Syntaxin 6 antibody [N1C3] (GTX115375) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for Syntaxin 6 antibody [N1C3] (GTX115375). Be the first to share your publications with this product.

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169