GeneTex
United States (US)

Syntaxin 6 antibody [N1C3]

GTX115375_40302_IHC_18121410_155.jpg
GTX115375_40302_WB_18121410_457.jpg
GTX115375_40302_20170706_WB_M_R_18121410_854.jpg
GTX115375_40302_20170727_IFA_R_18121410_683.jpg

Cat No. GTX115375

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Syntaxin 6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Syntaxin 6,Stx6

Cellular Localization

Golgi apparatus membrane; Single-pass type IV membrane protein

Background

Involved in intracellular vesicle trafficking.

Database

Research Area

DATA IMAGES
GTX115375_40302_IHC_18121410_155.jpg

GTX115375 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Syntaxin 6(GTX115375) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115375_40302_WB_18121410_457.jpg

GTX115375 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX115375 diluted at 1:1000

GTX115375_40302_20170706_WB_M_R_18121410_854.jpg

GTX115375 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntaxin 6 antibody [N1C3] (GTX115375) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115375_40302_20170727_IFA_R_18121410_683.jpg

GTX115375 ICC/IF Image

Syntaxin 6 antibody [N1C3] detects Syntaxin 6 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Syntaxin 6 protein stained by Syntaxin 6 antibody [N1C3] (GTX115375) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169