Syntenin 1 antibody [C2C3], C-term

Cat. No. GTX108470

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Syntenin 1 products

View product citations for antibody GTX108470 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Syntenin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037949

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

syndecan binding protein , MDA-9 , MDA9 , ST1 , SYCL , TACIP18

Cellular Localization

Cell junction , focal adhesion , adherens junction , Cell membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein , Nucleus , Melanosome , Cytoplasm , cytosol , cytoskeleton

Background

The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Gene ID: 6386 SDCBP
UniProt: O00560 SDCBP

Research Area

DATA IMAGES

GTX108470 WB Image

Syntenin 1 antibody detects Syntenin 1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody (GTX108470) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108470 IHC-Fr Image

Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse brain.
Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term (GTX108470) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX108470 IHC-P Image

Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Syntenin 1 antibody [C2C3], C-term (GTX108470) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108470 IHC-Fr Image

GTX108470 IHC-P Image

Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Syntenin 1 antibody [C2C3], C-term (GTX108470) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108470 IHC-P Image

GTX108470 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Syntenin 1(GTX108470) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX108470 WB Image

HeLa whole cell extracts and HeLa exosome extract (3.5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [C2C3], C-term (GTX108470) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Korvenlaita N et al. J Extracell Vesicles 2023; 12 (1) : e12297 Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia.

Haynes BA et al. Arterioscler Thromb Vasc Biol 2019; : ATVBAHA119312826 Endothelial-to-Mesenchymal Transition in Human Adipose Tissue Vasculature Alters the Particulate Secretome and Induces Endothelial Dysfunction.

Application : WB

Reactivity : Human

Kowal J et al. Proc Natl Acad Sci U S A 2016; 113 (8) : E968-77 Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes.

Application : WB

Reactivity : Human

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REVIEW

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Application Reference

Korvenlaita N et al. J Extracell Vesicles 2023; 12 (1):e12297 Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia.

Haynes BA et al. Arterioscler Thromb Vasc Biol 2019;:ATVBAHA119312826 Endothelial-to-Mesenchymal Transition in Human Adipose Tissue Vasculature Alters the Particulate Secretome and Induces Endothelial Dysfunction.

Application : WB

Reactivity : Human

Kowal J et al. Proc Natl Acad Sci U S A 2016; 113 (8):E968-77 Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes.

Application : WB

Reactivity : Human

SDS
Glycerol.pdf
Thimerosal.pdf

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty

Syntenin 1 antibody [C2C3], C-term

Cat. No. GTX108470

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX108470 WB Image

GTX108470 IHC-Fr Image

GTX108470 IHC-P Image

GTX108470 IHC-Fr Image

GTX108470 IHC-P Image

GTX108470 IHC-P Image

GTX108470 ICC/IF Image

GTX108470 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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