APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , rat hippocampus , HepG2 exosome extract
Predict Reactivity
Mouse, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Syntenin 1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888404
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
syndecan binding protein , MDA-9 , MDA9 , ST1 , SYCL , TACIP18
Cellular Localization
Cell junction , focal adhesion , adherens junction , Cell membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein , Nucleus , Melanosome , Cytoplasm , cytosol , cytoskeleton
Background
The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154](/templates/default/js/lightbox/images/loading.gif "Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154")
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GTX634154 WB Image
HepG2 whole cell and exosome extracts (1.8 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:250. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634154 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:5000.
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GTX634154 ICC/IF Image
Syntenin 1 antibody [GT1523] detects Syntenin 1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Syntenin 1 protein stained by Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX634154 WB Image
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
There are currently no references for Syntenin 1 antibody [GT1523] (GTX634154). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Syntenin 1 antibody [GT1523] (GTX634154). Be the first to share your experience with this product.
![Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154](/upload/website/prouct_img/small/GTX634154/GTX634154_42779_20190115_Fraction_19022001_733.jpg "Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154")
![Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154](/upload/website/prouct_img/small/GTX634154/GTX634154_42779_20170309_WB_18121411_816.jpg "Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154")
![Anti-Syntenin 1 antibody [GT1523] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634154](/upload/website/prouct_img/small/GTX634154/GTX634154_42779_20170824_IFA_18121411_602.jpg "Anti-Syntenin 1 antibody [GT1523] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634154")
![Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154](/upload/website/prouct_img/small/GTX634154/GTX634154_42779_20170922_WB_R_hippocampus_18121411_658.jpg "Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154")
![Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154](/upload/website/prouct_img/normal/GTX634154/GTX634154_42779_20190115_Fraction_19022001_733.jpg "Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154")
![Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154](/upload/website/prouct_img/normal/GTX634154/GTX634154_42779_20170309_WB_18121411_816.jpg "Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154")
![Anti-Syntenin 1 antibody [GT1523] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634154](/upload/website/prouct_img/normal/GTX634154/GTX634154_42779_20170824_IFA_18121411_602.jpg "Anti-Syntenin 1 antibody [GT1523] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634154")
![Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154](/upload/website/prouct_img/normal/GTX634154/GTX634154_42779_20170922_WB_R_hippocampus_18121411_658.jpg "Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154")