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Syntenin 1 antibody [GT1523]

Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154
Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154
Anti-Syntenin 1 antibody [GT1523] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634154
Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154

Cat No. GTX634154

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1523

Isotype

IgG2a

Application

WB, ICC/IF

Reactivity

Human, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

U87-MG , rat hippocampus

Predict Reactivity

Mouse, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Syntenin 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888404

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

syndecan binding protein , MDA-9 , MDA9 , ST1 , SYCL , TACIP18

Cellular Localization

Cell junction , focal adhesion , adherens junction , Cell membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein , Nucleus , Melanosome , Cytoplasm , cytosol , cytoskeleton

Background

The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154

GTX634154 WB Image

HepG2 whole cell and exosome extracts (1.8 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:250. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154

GTX634154 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:5000.

Anti-Syntenin 1 antibody [GT1523] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634154

GTX634154 ICC/IF Image

Syntenin 1 antibody [GT1523] detects Syntenin 1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Syntenin 1 protein stained by Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154

GTX634154 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169
Custom Conjugations