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Syntenin 1 antibody [GT1523]

Orthogonal
Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154
Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154
Anti-Syntenin 1 antibody [GT1523] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634154
Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154

Cat No. GTX634154

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1523

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($329),
25 μl ($169)
See all Syntenin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

U87-MG , rat hippocampus

Predict Reactivity

Mouse, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Syntenin 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

syndecan binding protein , MDA-9 , MDA9 , ST1 , SYCL , TACIP18

Cellular Localization

Cell junction , focal adhesion , adherens junction , Cell membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein , Nucleus , Melanosome , Cytoplasm , cytosol , cytoskeleton

Background

The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154

GTX634154 WB Image

Orthogonal

HepG2 whole cell and exosome extracts (1.8 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:250. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154

GTX634154 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:5000.

Anti-Syntenin 1 antibody [GT1523] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634154

GTX634154 ICC/IF Image

Syntenin 1 antibody [GT1523] detects Syntenin 1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Syntenin 1 protein stained by Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Syntenin 1 antibody [GT1523] used in Western Blot (WB). GTX634154

GTX634154 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169