APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , rat hippocampus , HepG2 exosome extract
Predict Reactivity
Mouse, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Syntenin 1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888404
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
syndecan binding protein , MDA-9 , MDA9 , ST1 , SYCL , TACIP18
Cellular Localization
Cell junction , focal adhesion , adherens junction , Cell membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein , Nucleus , Melanosome , Cytoplasm , cytosol , cytoskeleton
Background
The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX634154 WB Image
HepG2 whole cell and exosome extracts (1.8 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:250. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634154 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:5000.
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GTX634154 WB Image
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX634154 ICC/IF Image
Syntenin 1 antibody [GT1523] detects Syntenin 1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Syntenin 1 protein stained by Syntenin 1 antibody [GT1523] (GTX634154) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for Syntenin 1 antibody [GT1523] (GTX634154). Be the first to share your publications with this product.
REVIEW
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