*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Rat, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Syntenin 1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Syndecan Binding Protein,Mda-9,Mda9,St1,Sycl,Tacip18,Sdcbp
Cell junction , focal adhesion , adherens junction , Cell membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein , Nucleus , Melanosome , Cytoplasm , cytosol , cytoskeleton
The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
GTX108391 IHC-P Image
Syntenin 1 antibody [N1], N-term detects SDCBP protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Syntenin 1 antibody [N1], N-term (GTX108391) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX108391 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [N1], N-term (GTX108391) diluted at 1:500.
GTX108391 IP Image
Immunoprecipitation of Syntenin 1 protein from U87-MG whole cell extracts using 5 μg of Syntenin 1 antibody [N1], N-term (GTX108391).
Western blot analysis was performed using Syntenin 1 antibody [N1], N-term (GTX108391).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX108391 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Syntenin 1(GTX108391) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.