GeneTex
United States (US)

Syntenin 1 antibody [N1], N-term

GTX108391_39799_IHC_M_19011507_519.jpg
GTX108391_39799_20160310_WB_19011507_995.jpg
GTX108391_39799_20150717_IP_19011507_751.jpg
GTX108391_39799_IFA_19011507_466.jpg

Cat No. GTX108391

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ELISA
Reactivity Human, Mouse, Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Syntenin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Syndecan Binding Protein,Mda-9,Mda9,St1,Sycl,Tacip18,Sdcbp

Cellular Localization

Cell junction , focal adhesion , adherens junction , Cell membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein , Nucleus , Melanosome , Cytoplasm , cytosol , cytoskeleton

Background

The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108391_39799_IHC_M_19011507_519.jpg

GTX108391 IHC-P Image

Syntenin 1 antibody [N1], N-term detects SDCBP protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Syntenin 1 antibody [N1], N-term (GTX108391) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108391_39799_20160310_WB_19011507_995.jpg

GTX108391 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [N1], N-term (GTX108391) diluted at 1:500.

GTX108391_39799_20150717_IP_19011507_751.jpg

GTX108391 IP Image

Immunoprecipitation of Syntenin 1 protein from U87-MG whole cell extracts using 5 μg of Syntenin 1 antibody [N1], N-term (GTX108391).
Western blot analysis was performed using Syntenin 1 antibody [N1], N-term (GTX108391).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX108391_39799_IFA_19011507_466.jpg

GTX108391 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Syntenin 1(GTX108391) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169