APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human T-Plastin. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888315
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
plastin 3 , BMND18 , T-plastin
Background
Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]
Database
Research Area
DATA IMAGES
|
GTX632481 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with T-Plastin antibody [GT236] (GTX632481) diluted at 1:1000.
|
|
GTX632481 IHC-P Image
T-Plastin antibody [GT236] detects T-Plastin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.T-Plastin stained by T-Plastin antibody [GT236] (GTX632481) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX632481 IHC-P Image
T-Plastin antibody [GT236] detects T-Plastin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cervix.T-Plastin stained by T-Plastin antibody [GT236] (GTX632481) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX632481 WB Image
T-Plastin antibody detects T-Plastin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with T-Plastin antibody (GTX632481) diluted at a dilution of 1:1000.
|
REFERENCE
There are currently no references for T-Plastin antibody [GT236] (GTX632481). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for T-Plastin antibody [GT236] (GTX632481). Be the first to share your experience with this product.