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T-Plastin antibody

KO/KD Orthogonal
Anti-T-Plastin antibody used in Western Blot (WB). GTX103323
Anti-T-Plastin antibody used in Western Blot (WB). GTX103323
Anti-T-Plastin antibody used in Western Blot (WB). GTX103323
Anti-T-Plastin antibody used in Western Blot (WB). GTX103323
Anti-T-Plastin antibody used in IHC (Paraffin sections) (IHC-P). GTX103323
Anti-T-Plastin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103323

Cat. No. GTX103323

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all T-Plastin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , 293T , A431 , Jurkat , Raji

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human T-Plastin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951292

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

plastin 3 , BMND18 , T-plastin

Cellular Localization

Cytoplasm

Background

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-T-Plastin antibody used in Western Blot (WB). GTX103323

GTX103323 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with T-Plastin antibody (GTX103323) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-T-Plastin antibody used in Western Blot (WB). GTX103323

GTX103323 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with T-Plastin antibody (GTX103323) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-T-Plastin antibody used in Western Blot (WB). GTX103323

GTX103323 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX103323 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-T-Plastin antibody used in Western Blot (WB). GTX103323

GTX103323 WB Image

T-Plastin antibody detects T-Plastin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with T-Plastin antibody (GTX103323) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-T-Plastin antibody used in IHC (Paraffin sections) (IHC-P). GTX103323

GTX103323 IHC-P Image

T-Plastin antibody detects T-Plastin protein at cytoplasm on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
T-Plastin antibody (GTX103323) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-T-Plastin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103323

GTX103323 ICC/IF Image

T-Plastin antibody detects T-Plastin protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: T-Plastin protein stained by T-Plastin antibody (GTX103323) diluted at 1:100.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Eshraghi M et al. Hum Mol Genet 2016; 25 (20) : 4494-4506. Effect of genetic background on the phenotype of the Smn2B/- mouse model of spinal muscular atrophy.
Application : WB
Reactivity : Mouse
van Dijk FS et al. N Engl J Med 2013; 369 (16) : 1529-36 PLS3 mutations in X-linked osteoporosis with fractures.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169