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T1R3 antibody [C1C2], Internal

Orthogonal
Anti-T1R3 antibody [C1C2], Internal used in Western Blot (WB). GTX116069

Cat. No. GTX116069

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all T1R3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

K562 , K562 MF

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human T1R3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621278

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

taste 1 receptor member 3 , T1R3

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is a G-protein coupled receptor involved in taste responses. The encoded protein can form a heterodimeric receptor with TAS1R1 to elicit the umami taste response, or it can bind with TAS1R2 to form a receptor for the sweet taste response. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-T1R3 antibody [C1C2], Internal used in Western Blot (WB). GTX116069

GTX116069 WB Image

Orthogonal

T1R3 antibody [C1C2] detects T1R3 protein by western blot analysis. K562 whole cell extracts and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with T1R3 antibody [C1C2] (GTX116069) at a dilution of 1:500.

REFERENCE

Application Reference

Meyer D et al. PLoS One 2012; 7 (2) : e32354 Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca2+ and cAMP concentrations in spermatozoa.
Application : ICC/IF
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169