T7 tag antibody detects T7 tag protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of T7-tagged protein expressing 293T cells
12% SDS-PAGE
T7 tag antibody (GTX128059) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.