ELISA: Use at a dilution of: Coating: 1/100 - 1/500Primary/ 1:1000 - 1/30,000. ICC: Use at a dilution of 1/200 - 1/2000. WB: Use at a dilution of 1/1000 - 1/30000. Optimal dilutions/concentrations should be determined by the end user.
This antibody was verified by ELISA against peptide conjugated to BSA(MASMTGGQQMG/BSA). Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
PBS, pH 7.2, sodium azide, 0.1%
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
1mg/ml(Please refer to the vial label for the specific concentration.)
Synthetic peptide: MASMTGGQQMG (T7) conjugated to KLH.
Immunogen affinity purified
This antibody was affinity purified using the peptide immobilized on solid support..all
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.