GeneTex
United States (US)

TAB1 antibody

GTX107571_39946_IHC_19011506_617.jpg
GTX107571_39946_WB_M_19011506_524.jpg
GTX107571_IFA_19011506_335.jpg
GTX107571_39946_WB_19011506_655.jpg

Cat No. GTX107571

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , A431 , HeLa , A375 , GL261 , C8D30

Predict Reactivity

Rat, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TAB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tgf-Beta Activated Kinase 1 (Map3K7) Binding Protein 1,3'-Tab1,Map3K7Ip1,Tab1

Background

The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107571_39946_IHC_19011506_617.jpg

GTX107571 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TAB1(GTX107571) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107571_39946_WB_M_19011506_524.jpg

GTX107571 WB Image

TAB1 antibody detects MAP3K7IP1 protein by western blot analysis.
A. 30 µg GL261 whole cell lysate/extract
B. 30 µg C8D30 whole cell lysate/extract
10% SDS-PAGE
TAB1 antibody (GTX107571) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107571_IFA_19011506_335.jpg

GTX107571 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAB1(GTX107571) antibody at 1:100 dilution.

GTX107571_39946_WB_19011506_655.jpg

GTX107571 WB Image

TAB1 antibody detects MAP3K7IP1 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg A375 whole cell lysate/extract
10% SDS-PAGE
TAB1 antibody (GTX107571) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169