GeneTex
United States (US)

TAB1 antibody

GTX107892_40009_20160728_WB_R_19011507_760.jpg
GTX107892_WB_19011507_779.jpg
GTX107892_40009_20160728_WB_M_19011507_613.jpg
GTX107892_IFA_19011507_762.jpg

Cat No. GTX107892

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A431 , Molt-4 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TAB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tgf-Beta Activated Kinase 1 (Map3K7) Binding Protein 1,3'-Tab1,Map3K7Ip1,Tab1

Background

The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107892_40009_20160728_WB_R_19011507_760.jpg

GTX107892 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TAB1 antibody (GTX107892) diluted at 1:500.

GTX107892_WB_19011507_779.jpg

GTX107892 WB Image

Sample (30μg whole cell lysate)
A:A431(GTX27909)
B:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX107892 diluted at 1:1000

GTX107892_40009_20160728_WB_M_19011507_613.jpg

GTX107892 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TAB1 antibody (GTX107892) diluted at 1:500.

GTX107892_IFA_19011507_762.jpg

GTX107892 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAB1(GTX107892) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169