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TAF1 antibody

Anti-TAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00900
Anti-TAF1 antibody used in Western Blot (WB). GTX00900
Anti-TAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00900

Cat No. GTX00900

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat, Hamster
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:1000-1:5000
Not tested in other applications.

Calculated MW

213 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum with 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human TFIID subunit 1 protein corresponding to amino acids 103-123, STEDAVDYSDINEVAEDESRR-C, conjugated with KLH

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TATA-box binding protein associated factor 1 , BA2R , CCG1 , CCGS , DYT3 , DYT3/TAF1 , KAT4 , MRXS33 , NSCL2 , NTAF1 , OF , P250 , TAF(II)250 , TAF1 , TAF2A , TAFII250 , TATAbox binding protein associated factor 1 , XDP , TBP Associated Factor 1

Cellular Localization

Nucleus

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]

Database

Research Area

DATA IMAGES

Anti-TAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00900

GTX00900 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00900 TAF1 antibody. TAF1 protein was detected in nucleus.
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100
Dilution : 1:5000

Anti-TAF1 antibody used in Western Blot (WB). GTX00900

GTX00900 WB Image

WB analysis of HeLa whole cell lysate (10 μg) using GTX00900 TAF1 antibody.

Anti-TAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00900

GTX00900 ICC/IF Image

ICC/IF analysis of BHK cells using GTX00900 TAF1 antibody. TAF1 antibody pre-incubated with (right panel) or without (left panel) immunogen peptide.
Fixation : 3% formaldehyde
Permeabilization : 1% NP-40

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 369