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TAF1 antibody

Anti-TAF1 antibody used in ChIP assay (ChIP assay). GTX48659
Anti-TAF1 antibody used in ChIP assay (ChIP assay). GTX48659
Anti-TAF1 antibody used in Western Blot (WB). GTX48659

Cat. No. GTX48659

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ELISA, ChIP assay

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all TAF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:2000
IHC-P 10 μg/mL
IP Assay dependent
ELISA 1:150000-1:300000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

213 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acids near the carboxyl terminus of human TAF1.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

RRID

AB_11171844

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TATA-box binding protein associated factor 1 , BA2R , CCG1 , CCGS , DYT3 , DYT3/TAF1 , KAT4 , MRXS33 , N-TAF1 , NSCL2 , OF , P250 , TAF(II)250 , TAF2A , TAFII-250 , TAFII250 , XDP

Cellular Localization

Nucleus

Background

Transcription Initiation Factor TFIID Subunit 1 (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex, which also includes TATA-binding protein (TBP) and a variety of TBP-associated factors. TFIID nucleates the formation of transcription pre-initiation complexes and plays a key role in the regulation of gene expression by RNA polymerase II. TAF1 possesses DNA-binding activity and also contains novel N- and C-terminal Ser/Thr kinase domains which can auto-phosphorylate or trans-phosphorylate other transcription factors. For example, TAF1 interacts with the C-terminus of TP53 and phosphorylates the T55 residue, leading to MDM2-mediated degradation of TP53. TAF1 also catalyzes Ser phosphorylation of general transcription factor IIA (GTF2A1) and IIF (GTF2F1). The retinoblastoma tumor suppressor protein, RB1, interacts with the N-terminal domain of TAF1 and inhibits its intrinsic kinase activity. TAF1 is essential for progression through the G1 phase of the cell cycle and has been reported to be indispensable for expression of 18% of mammalian genes.

Database

Research Area

DATA IMAGES

Anti-TAF1 antibody used in ChIP assay (ChIP assay). GTX48659

GTX48659 ChIP assay Image

Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody (GTX48659) was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). ChIP was performed on fos promoters using 5 μg of TAF1 antibody(GTX48659) and an RNA PolII antibody.

Anti-TAF1 antibody used in ChIP assay (ChIP assay). GTX48659

GTX48659 ChIP assay Image

Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody (GTX48659) was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). ChIP was performed on fos promoters using 5 μg of TAF1 antibody(GTX48659) and an RNA PolII antibody.

Anti-TAF1 antibody used in Western Blot (WB). GTX48659

GTX48659 WB Image

WB analysis of various samples using GTX48659 TAF1 antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : A431 whole cell lysate
Lane 6 : A549 whole cell lysate
Lane 7 : LNCap whole cell lysate
Lane 8 : Molt-4 whole cell lysate
Lane 9 : Ramos whole cell lysate
Lane 10 : Raji whole cell lysate
Lane 11 : A-172 whole cell lysate
Lane 12 : NIH-3T3 whole cell lysate
Loading : 35 μg
Dilution : 0.2 μg/mL

REFERENCE

Application Reference

Zhang H et al. Cell Mol Neurobiol 2020; Valproate-Induced Epigenetic Upregulation of Hypothalamic Fto Expression Potentially Linked with Weight Gain.
Application : IP
Reactivity : Mouse
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399