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TAF1 antibody

Anti-TAF1 antibody used in ChIP assay (ChIP assay). GTX48659
Anti-TAF1 antibody used in ChIP assay (ChIP assay). GTX48659

Cat No. GTX48659

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

IP, ELISA, ChIP assay, IHC

Reactivity

Human, Mouse, Rat
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IP Assay dependent
ELISA 1:150000 - 1:300000
ChIP assay Assay dependent
IHC 10μg/ml
Not tested in other applications.

Calculated MW

213 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human TAF1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TATA-box binding protein associated factor 1 , BA2R , CCG1 , CCGS , DYT3 , DYT3/TAF1 , KAT4 , MRXS33 , N-TAF1 , NSCL2 , OF , P250 , TAF(II)250 , TAF2A , TAFII-250 , TAFII250 , XDP

Cellular Localization

Nucleus

Background

Transcription Initiation Factor TFIID Subunit 1 (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex, which also includes TATA-binding protein (TBP) and a variety of TBP-associated factors. TFIID nucleates the formation of transcription pre-initiation complexes and plays a key role in the regulation of gene expression by RNA polymerase II. TAF1 possesses DNA-binding activity and also contains novel N- and C-terminal Ser/Thr kinase domains which can auto-phosphorylate or trans-phosphorylate other transcription factors. For example, TAF1 interacts with the C-terminus of TP53 and phosphorylates the T55 residue, leading to MDM2-mediated degradation of TP53. TAF1 also catalyzes Ser phosphorylation of general transcription factor IIA (GTF2A1) and IIF (GTF2F1). The retinoblastoma tumor suppressor protein, RB1, interacts with the N-terminal domain of TAF1 and inhibits its intrinsic kinase activity. TAF1 is essential for progression through the G1 phase of the cell cycle and has been reported to be indispensable for expression of 18% of mammalian genes.

Database

Research Area

DATA IMAGES

Anti-TAF1 antibody used in ChIP assay (ChIP assay). GTX48659

GTX48659 ChIP assay Image

Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody (GTX48659) was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). ChIP was performed on fos promoters using 5 μg of TAF1 antibody(GTX48659) and an RNA PolII antibody.

Anti-TAF1 antibody used in ChIP assay (ChIP assay). GTX48659

GTX48659 ChIP assay Image

Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody (GTX48659) was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). ChIP was performed on fos promoters using 5 μg of TAF1 antibody(GTX48659) and an RNA PolII antibody.

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf