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TAF15 antibody [N1N3]

Anti-TAF15 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103116
Anti-TAF15 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103116
Anti-TAF15 antibody [N1N3] used in Western Blot (WB). GTX103116
Anti-TAF15 antibody [N1N3] used in Western Blot (WB). GTX103116
Anti-TAF15 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103116

Cat No. GTX103116

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TAF15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TATA-box binding protein associated factor 15 , Npl3 , RBP56 , TAF2N , TAFII68

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-TAF15 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103116

GTX103116 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using TAFII68(GTX103116) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TAF15 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103116

GTX103116 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAFII68 (GTX103116) antibody at 1:200 dilution.

Anti-TAF15 antibody [N1N3] used in Western Blot (WB). GTX103116

GTX103116 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
7.5% SDS PAGE
GTX103116 diluted at 1:1000

Anti-TAF15 antibody [N1N3] used in Western Blot (WB). GTX103116

GTX103116 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX103116 diluted at 1:1000

Anti-TAF15 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103116

GTX103116 IHC-P Image

TAFII68 antibody [N1N3] detects TAFII68 protein at nucleus in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TAFII68 antibody [N1N3] (GTX103116) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169