GeneTex
United States (US)

TAF172 antibody [C3], C-term

GTX106359_39946_IP_19011506_748.jpg
GTX106359_WB_19011506_270.jpg

Cat No. GTX106359

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

207 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TAF172. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

KIAA0940 antibody, MGC138406 antibody, MOT1 antibody, TAF(II)170 antibody, TAF172 antibody, TAFII170 antibody, BTAF1 antibody, B-TFIID transcription factor-associated 170 kDa subunit antibody, ATP-dependent helicase BTAF1 antibody, TATA-binding protein-associated factor 172 antibody, TBP-associated factor 172 antibody, BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae) antibody

Cellular Localization

Nucleus

Background

Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP; MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B; MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX106359_39946_IP_19011506_748.jpg

GTX106359 IP Image

TAF172 antibody immunoprecipitates TAF172 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TAF172 protein by 2 μg of TAF172 antibody (GTX106359) 5% SDS-PAGE The immunoprecipitated TAF172 protein was detected by TAF172 antibody (GTX106359) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX106359_WB_19011506_270.jpg

GTX106359 WB Image

Sample(30μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
5% SDS PAGE
GTX106359 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169