TAF172 antibody

Cat. No. GTX30095

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP, ChIP assay
Reactivity	Human, Rat
Package	100 μl ($399)

See all TAF172 products

Datasheet
Document

View product citations for antibody GTX30095 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
IP	1:20 - 1:50
ChIP assay	1:20 - 1:100

Not tested in other applications.

Calculated MW

207 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1600-1849 of human BTAF1 (NP_003963.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

B-TFIID TATA-box binding protein associated factor 1 , MOT1 , TAF(II)170 , TAF172 , TAFII170

Cellular Localization

Nucleus

Background

This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters. [provided by RefSeq, Sep 2011]

Database

Gene ID: 9044 BTAF1
UniProt: O14981 BTAF1

Research Area

DATA IMAGES

GTX30095 WB Image

WB analysis of various sample lysates using GTX30095 TAF172 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX30095 ChIP assay Image

ChIP analysis of HeLa cell lysate using GTX30095 TAF172 antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

GTX30095 IP Image

IP analysis of HeLa cell lysate using GTX30095 TAF172 antibody.
Antibody amount : 3μg / 150μg lysate
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty