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TAF5 antibody

Anti-TAF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32908
Anti-TAF5 antibody used in Western Blot (WB). GTX32908

Cat No. GTX32908

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 656-800 of human TAF5 (NP_008882.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TATA-box binding protein associated factor 5 , TAF(II)100 , TAF2D , TAFII-100 , TAFII100

Cellular Localization

Nucleus

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-TAF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32908

GTX32908 ICC/IF Image

ICC/IF analysis of MCF-7 cell using GTX32908 TAF5 antibody.
Blue : DAPI

Anti-TAF5 antibody used in Western Blot (WB). GTX32908

GTX32908 WB Image

WB analysis of mouse brain tissue lysate using GTX32908 TAF5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 329