GeneTex
United States (US)

TAF7 antibody

GTX105174_40506_IP_19011506_342.jpg
GTX105174_40506_WB_19011506_550.jpg
GTX105174_40506_WB_2_19011506_643.jpg

Cat No. GTX105174

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

A431 , JurkatHeLa , HepG2 , A431 , Jurkat

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TAF7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TAF2F antibody, TAFII55 antibody, TAF7 antibody, TAFII-55 antibody, RNA polymerase II TBP-associated factor subunit F antibody, TATA box binding protein (TBP)-associated factor, RNA polymerase II, F, 55kD antibody, transcription factor IID subunit TAFII55 antibody, transcription initiation factor TFIID 55 kDa subunit antibody, TBP-associated factor F antibody, transcription initiation factor TFIID, 55 kDa subunit antibody, transcription initiation factor TFIID subunit 7 antibody, TAF(II)55 antibody, TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa antibody

Cellular Localization

Nucleus

Background

The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II. [provided by RefSeq]

Database

DATA IMAGES
GTX105174_40506_IP_19011506_342.jpg

GTX105174 IP Image

TAF7 antibody immunoprecipitates TAF7 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TAF7 protein by 2 μg of TAF7 antibody (GTX105174) 7.5% SDS-PAGE The immunoprecipitated TAF7 protein was detected by TAF7 antibody (GTX105174) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX105174_40506_WB_19011506_550.jpg

GTX105174 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: Jurkat
10% SDS PAGE
GTX105174 diluted at 1:1000

GTX105174_40506_WB_2_19011506_643.jpg

GTX105174 WB Image

TAF7 antibody detects TAF7 protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
TAF7 antibody (GTX105174) dilution: 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169