APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89337-PEP).
Product Note
This antibody is expected to recognise isoform A (NP_003179.1) and isoform B (NP_663304.1).
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-AELDQDEKDQQNT, from the internal region of the protein sequence according to NP_003179.1; NP_663304.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase kinase kinase 7 , CSCF , FMD2 , MEKK7 , TAK1 , TGF1a
Cellular Localization
Cytoplasm , Cell membrane
Background
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX89337 WB Image
WB analysis of HEK293 overexpressing human TAK1 with DYKDDDDK tag using GTX89337 TAK1 antibody, Internal.
Lane A : primary antibody
Lane B : Mock-transfected HEK293 probed with primary antibody
Lane C : Tag antibody
Dilution : 1μg/ml
Loading : 10μg protein in RIPA buffer
|
REFERENCE
There are currently no references for TAK1 antibody, Internal (GTX89337). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TAK1 antibody, Internal (GTX89337). Be the first to share your experience with this product.
