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TAK1 (phospho Thr184) antibody

Orthogonal
Anti-TAK1 (phospho Thr184) antibody used in Western Blot (WB). GTX55449
Anti-TAK1 (phospho Thr184) antibody used in Western Blot (WB). GTX55449

Cat. No. GTX55449

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all TAK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 184 (I-Q-T(p)-H-M) derived from human TAK1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_2887928

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 7 , CSCF , FMD2 , MEKK7 , TAK1 , TGF1a

Cellular Localization

Cytoplasm,Cell membrane

Background

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TAK1 (phospho Thr184) antibody used in Western Blot (WB). GTX55449

GTX55449 WB Image

Orthogonal

WB analysis of extracts from HepG2 cells treated with TNF (20ng/ml 5mins) using GTX55449 TAK1 (phospho Thr184) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-TAK1 (phospho Thr184) antibody used in Western Blot (WB). GTX55449

GTX55449 WB Image

WB analysis of extracts from 293 cells (lane 2) using GTX55449 TAK1 (phospho Thr184) antibody.
Left : Primary antibody pre-incubated with the antigen specific peptide
Right : Primary antibody

REFERENCE

Application Reference

Chang HH et al. Aging (Albany NY) 2020; 12 (21) : 21253-21272 Regulation of the regenerative activity of dental pulp stem cells from exfoliated deciduous teeth (SHED) of children by TGF-β1 is associated with ALK5/Smad2, TAK1, p38 and MEK/ERK signaling.
Application : WB
Reactivity : Human
Chang MC et al. J Tissue Eng Regen Med 2016; (Epub) Effects of TGF-β1 on plasminogen activation in human dental pulp cells: Role of ALK5/Smad2, TAK1 and MEK/ERK signaling.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399