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TAL1 antibody [C2C3], C-term

Orthogonal
Anti-TAL1 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX116020
Anti-TAL1 antibody [C2C3], C-term used in Western Blot (WB). GTX116020
Anti-TAL1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116020
Anti-TAL1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116020

Cat. No. GTX116020

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TAL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TAL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038090

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TAL bHLH transcription factor 1, erythroid differentiation factor , SCL , TCL5 , bHLHa17 , tal-1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-TAL1 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX116020

GTX116020 IP Image

TAL1 antibody immunoprecipitates TAL1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TAL1 protein by 2 μg of TAL1 antibody (GTX116020) 12% SDS-PAGE The immunoprecipitated TAL1 protein was detected by TAL1 antibody (GTX116020) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TAL1 antibody [C2C3], C-term used in Western Blot (WB). GTX116020

GTX116020 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TAL1 antibody [C2C3], C-term (GTX116020) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-TAL1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116020

GTX116020 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft , using TAL1(GTX116020) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TAL1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116020

GTX116020 IHC-P Image

TAL1 antibody [C2C3], C-term detects TAL1 protein at cytosol and nucleus on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
TAL1 antibody [C2C3], C-term (GTX116020) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

C Collart et al. BMC Dev Biol 2021; 21 (1) : 8 The migratory pathways of the cells that form the endocardium, dorsal aortae, and head vasculature in the mouse embryo.
Reactivity : Mouse
Qi Q et al. Blood 2021; 137 (10) : 1327-1339 Dynamic CTCF binding directly mediates interactions among cis-regulatory elements essential for hematopoiesis.
Application : ChIP assay
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169