GeneTex
United States (US)

TAP antibody [N1N2], N-term

GTX102071_IHC_19011506_974.jpg
GTX102071_WB_19011506_270.jpg
GTX102071_IFA_19011506_840.jpg

Cat No. GTX102071

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

HeLa , HepG2 , Molt-4

Predict Reactivity

Mouse, Rat, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Rna Export Factor 1,Mex67,Tap,Nxf1

Cellular Localization

Nucleus , nucleoplasm , Nucleus speckle , Cytoplasm

Background

This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

DATA IMAGES
GTX102071_IHC_19011506_974.jpg

GTX102071 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft , using TAP (GTX102071) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102071_WB_19011506_270.jpg

GTX102071 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX102071 diluted at 1:1000

GTX102071_IFA_19011506_840.jpg

GTX102071 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAP (GTX102071) antibody at 1:200 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169