APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , Molt-4
Predict Reactivity
Mouse, Rat, Pig, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.3 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human TAP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2037558
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear RNA export factor 1 , MEX67 , TAP
Cellular Localization
Nucleus , nucleoplasm , Nucleus speckle , Cytoplasm
Background
This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX102071 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft , using TAP (GTX102071) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102071 WB Image
Sample (30 ug of whole cell lysate) A: Hela B: Molt-4 (GTX27912) 7.5% SDS PAGE GTX102071 diluted at 1:1000
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GTX102071 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAP (GTX102071) antibody at 1:200 dilution.
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REFERENCE
There are currently no references for TAP antibody [N1N2], N-term (GTX102071). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TAP antibody [N1N2], N-term (GTX102071). Be the first to share your experience with this product.