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TAZ antibody

Anti-TAZ antibody used in Western Blot (WB). GTX134857
Anti-TAZ antibody used in Western Blot (WB). GTX134857
Anti-TAZ antibody used in Western Blot (WB). GTX134857
Anti-TAZ antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134857
Anti-TAZ antibody used in Western Blot (WB). GTX134857
Anti-TAZ antibody used in IHC (Paraffin sections) (IHC-P). GTX134857

Cat. No. GTX134857

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TAZ products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TAZ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887361

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WW domain containing transcription regulator 1 , TAZ

Cellular Localization

Nucleus,Cytoplasm

Database

Research Area

DATA IMAGES

Anti-TAZ antibody used in Western Blot (WB). GTX134857

GTX134857 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TAZ antibody (GTX134857) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TAZ antibody used in Western Blot (WB). GTX134857

GTX134857 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TAZ antibody (GTX134857) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TAZ antibody used in Western Blot (WB). GTX134857

GTX134857 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TAZ antibody (GTX134857) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TAZ antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134857

GTX134857 ICC/IF Image

TAZ antibody detects TAZ protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TAZ stained by TAZ antibody (GTX134857) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TAZ antibody used in Western Blot (WB). GTX134857

GTX134857 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TAZ antibody (GTX134857) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TAZ antibody used in IHC (Paraffin sections) (IHC-P). GTX134857

GTX134857 IHC-P Image

TAZ antibody detects TAZ protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
TAZ stained by TAZ antibody (GTX134857) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169