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TBCA antibody [AT1A5]

Anti-TBCA antibody [AT1A5] used in Western Blot (WB). GTX50016

Cat No. GTX50016

Host

Mouse

Clonality

Monoclonal

Clone Name

AT1A5

Isotype

IgG2b

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1000 ~ 2000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant TBCA (1-108aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Tubulin Folding Cofactor A , Tbca

Background

Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest.

Database

Research Area

DATA IMAGES

Anti-TBCA antibody [AT1A5] used in Western Blot (WB). GTX50016

GTX50016 WB Image

Cell lysates of LNCaP(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TBCA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf