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TBP antibody [1TBP18]

Anti-TBP antibody [1TBP18] used in Western Blot (WB). GTX20818

Cat No. GTX20818

Host

Mouse

Clonality

Monoclonal

Clone Name

1TBP18

Isotype

IgG1

Application

WB, ICC/IF, IP, Gel supershift assays

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IP Assay dependent
Gel supershift assays Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Product Note

This antibody recognizes an epitope within amino acid residues 1-20 of human, mouse and rat TBP.
We do not recommend use of this product for Yeast,Drosophila,Silk worm,Xenopus samples.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human TBP.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_384537

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GTF2D , GTF2D1 , HDL4 , SCA17 , TATAbox binding protein , TBP , TFIID , TATA binding protein TBP , TATA box binding protein , TATA-box binding protein

Cellular Localization

Nucleus

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-TBP antibody [1TBP18] used in Western Blot (WB). GTX20818

GTX20818 WB Image

WB analysis of ecombinant human TATA Binding Protein, HeLa cell nuclear extract (A), and TBP protein fragment lysate (B) using GTX20818 TBP antibody [1TBP18].
Dilution : 2μg/ml

REFERENCE

REVIEW

TBP antibody [1TBP18] Cat No. GTX20818

Rating ( Average 5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25549101
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25549101
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25549101
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25549101
SDS
PBS.pdf
Package List Price ($)
$ 339