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TBP antibody [4H2]

Anti-TBP antibody [4H2] used in Western Blot (WB). GTX34253

Cat No. GTX34253

Host Mouse
Clonality Monoclonal
Clone Name 4H2
Isotype IgG1
Application WB
Reactivity Human, Mouse, Rat
See all TBP products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Specificity/Sensitivity

TBP/TATA Binding Protein Mouse Monoclonal Antibody (4H2) detects endogenous levels of TBP/TATA Binding Protein
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant Protein

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tata-Box Binding Protein,Gtf2D,Gtf2D1,Hdl4,Sca17,Tfiid,Tbp

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of trans

Database

Research Area

DATA IMAGES
Anti-TBP antibody [4H2] used in Western Blot (WB). GTX34253

GTX34253 WB Image

Western Blot (WB) analysis of 1) HeLa, 2)Mouse Brain Tissue, 3) Rat Brain Tissue with TBP/TATA Binding Protein Mouse Monoclonal Antibody diluted at 1:2000.
Package List Price ($)
$ 319