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TBP antibody

Anti-TBP antibody used in Western Blot (WB). GTX133204
Anti-TBP antibody used in Western Blot (WB). GTX133204
Anti-TBP antibody used in IHC (Paraffin sections) (IHC-P). GTX133204
Anti-TBP antibody used in IHC (Paraffin sections) (IHC-P). GTX133204
Anti-TBP antibody used in IHC (Paraffin sections) (IHC-P). GTX133204

Cat No. GTX133204

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chicken, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Human TBP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TATA-box binding protein,GTF2D,GTF2D1,HDL4,SCA17,TFIID

Cellular Localization

Nucleus

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-TBP antibody used in Western Blot (WB). GTX133204

GTX133204 WB Image

Wild-type (WT) and TBP knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TBP antibody (GTX133204) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TBP antibody used in Western Blot (WB). GTX133204

GTX133204 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TBP antibody (GTX133204) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TBP antibody used in IHC (Paraffin sections) (IHC-P). GTX133204

GTX133204 IHC-P Image

TBP antibody detects TBP protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
TBP stained by TBP antibody (GTX133204) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TBP antibody used in IHC (Paraffin sections) (IHC-P). GTX133204

GTX133204 IHC-P Image

TBP antibody detects TBP protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
TBP stained by TBP antibody (GTX133204) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TBP antibody used in IHC (Paraffin sections) (IHC-P). GTX133204

GTX133204 IHC-P Image

TBP antibody detects TBP protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
TBP stained by TBP antibody (GTX133204) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf