GeneTex
United States (US)

TBR1 antibody [N2C1], Internal

GTX117615_40779_20180131_ICC_IF_R_18121410_309.jpg
GTX117615_40779_WB_M_18121410_404.jpg
GTX117615_40779_20161110_IHC-P_M_18121410_492.jpg

Cat No. GTX117615

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

Neuro2A , GL261

Predict Reactivity

Human, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TBR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC141978 antibody, TBR-1 antibody, TES-56 antibody, TBR1 antibody, T-box brain protein 1 antibody, T-brain-1 antibody, "T-box, brain, 1 antibody"

Cellular Localization

Nucleus

Background

This gene is a member of a conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similar protein has been disrupted in mice and shown to be critical for early cortical development, and causes loss of projection neurons in the olfactory bulbs and olfactory cortex. The C-terminal region this similar protein was found to be necessary and sufficient for association with the guanylate kinase domain of calcium/calmodulin-dependent serine protein kinase. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX117615_40779_20180131_ICC_IF_R_18121410_309.jpg

GTX117615 ICC/IF Image

TBR1 antibody [N2C1], Internal detects TBR1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TBR1 stained by TBR1 antibody [N2C1], Internal (GTX117615) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX117615_40779_WB_M_18121410_404.jpg

GTX117615 WB Image

Sample (30 ug of whole cell lysate)
A: Neuro2A
B: GL261
7.5% SDS PAGE
GTX117615 diluted at 1:1000

GTX117615_40779_20161110_IHC-P_M_18121410_492.jpg

GTX117615 IHC-P Image

TBR1 antibody [N2C1], Internal detects TBR1 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TBR1 antibody [N2C1], Internal (GTX117615) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf