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TBR1 antibody

Anti-TBR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133228
Anti-TBR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133228
Anti-TBR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133228
Anti-TBR1 antibody used in Western Blot (WB). GTX133228

Cat No. GTX133228

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

mouse brain

Predict Reactivity

Human, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of mouse TBR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

T-box brain gene 1

Database

Research Area

DATA IMAGES
Anti-TBR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133228

GTX133228 IHC-P Image

TBR1 antibody detects TBR1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TBR1 stained by TBR1 antibody (GTX133228) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TBR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133228

GTX133228 ICC/IF Image

TBR1 antibody detects TBR1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TBR1 stained by TBR1 antibody (GTX133228) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-TBR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133228

GTX133228 IHC-P Image

TBR1 antibody detects TBR1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TBR1 stained by TBR1 antibody (GTX133228) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TBR1 antibody used in Western Blot (WB). GTX133228

GTX133228 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TBR1 antibody (GTX133228) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169