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TBX21 antibody

Anti-TBX21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31554
Anti-TBX21 antibody used in Western Blot (WB). GTX31554

Cat. No. GTX31554

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all TBX21 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

HEK293 Cell Slide(GTX25524) , HEK293 Cell Lysate(GTX27902)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TBX21 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human TBX2.The immunogen is located within the last 50 amino acids of TBX21 .

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

T-box 21 , T-PET , T-bet , TBET , TBLYM

Cellular Localization

Nucleus

Background

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TBX21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31554

GTX31554 ICC/IF Image

ICC/IF analysis of 293 cells using GTX31554 TBX21 antibody.
Working concentration : 5 μg/ml

Anti-TBX21 antibody used in Western Blot (WB). GTX31554

GTX31554 WB Image

WB analysis of 293 cell lysate using GTX31554 TBX21 antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399