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TBX21 antibody

Anti-TBX21 antibody used in Western Blot (WB). GTX31554
Anti-TBX21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31554

Cat No. GTX31554

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

HEK293 Cell Slide(GTX25524) , HEK293 Cell Lysate(GTX27902)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TBX21 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human TBX2.The immunogen is located within the last 50 amino acids of TBX21 .

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

T-box 21 , T-PET , T-bet , TBET , TBLYM

Cellular Localization

Nucleus

Background

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TBX21 antibody used in Western Blot (WB). GTX31554

GTX31554 WB Image

WB analysis of 293 cell lysate using GTX31554 TBX21 antibody.
Working concentration : 1 μg/ml

Anti-TBX21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31554

GTX31554 ICC/IF Image

ICC/IF analysis of 293 cells using GTX31554 TBX21 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339